Interference of ADSCs in the activity of advanced glycation end products (AGE) on cultured fibroblasts from diabetic wounds

• Grant number: 12/00343-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2012
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Cesar Isaac
• Grantee: Pedro Ribeiro Soares de Ladeira
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the diabetic chronic ulcer repair process, the fail of wound repair is associated to abnormal cellular and molecular response at all stages of the wound healing. Known factors such as dermal accumulation of advanced glycation end products (advanced glycation end products - AGEs) justify the chronicity of those lesions in diabetic patients. In the wound matrix, the protein glycation is initiated spontaneously by non-enzymatic reactions between free amino groups and carbonyl groups of sugars. ADSCs are stem cells obtained from the stroma of white adipose tissue which produces biologically active substances that interfere in the production of collagen, fibronectin, VEGF and FGF. The present study was designed to evaluate the in vitro ability of ADSCs modulate the activity of advanced glycation end products (AGEs) in cultured fibroblasts from granulation tissue of wounds present in the lower limbs of diabetic individuals, which possibly could become a new therapeutic goal in this pathology. (AU)