EVALUATION OF PERITONEAL REACTION AFTER SMALL COLON ENTEROTOMY IN TREATED HORSES WITH HEPARIN

• Grant number: 12/03589-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2012
• End date: July 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Carlos Alberto Hussni
• Grantee: Juliana de Moura Alonso
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Adhesion formation in equine occurs as a result of peritoneal inflammation, being responsibleto the main postoperative complication in abdominal surgery, resulting in chronic pain,bowell oclusion or sub-oclusion, is essential to develop research that act in a mannerappropriate adjuvant to surgical techniques in the inhibition of adhesion. The objective is toevaluate the effect of sodic heparin(SH) in inhibiting inflammatory reaction and abdominaladhesions in horses. Eight horses will be used, subject to enterotomy and abrasion in seromuscularlayer of the small colon trough the right flank laparotomy (P1) animals will bedivided into two groups of four animals, group 1, control (enterotomy + peritoneal abrasion:E+ PA), for group 2 (E+PA and SH at a dose of 150 UI/Kg subcutaneously every 12 hours,during 5 days, from P1). Previously to the P1(A0), 12 hours after P1(A1), 24 hours afterP1(A2), 48 hours after P1(A3), 96 hours after P1(A4) and 144 hours after P1(A5) will becollected : venous blood for hemograma, plasma fibrinogen, coagulogram and acute phaseproteins (AFP); peritoneal fluid to assess physical, chemical, cytological, ELISA for tissueplasminogen activator (tPA) and plasminogen activator inhibitor (PAI-1), latex agglutinationtest for D-dimer and measurement of AFP. After 15 days of P1 laparoscopy will be performed(P2) in order to evaluate the effectiveness of treatment. Although there are many researchsavailable in the literature addressing the use of heparin in inhibiting the formation ofadhesions, there is no consensus in the equine species, being necessary to carry out studies toassess this use. Is expected that these drug inhibit peritoneal reaction and adhesionsformation, resulting in reduction of postoperative complications and increased survival rate ofhorses undergoing laparotomy.