Selection of candidate species and evaluation of its larval density for use in larval therapy in order to recover tegumentary injuries

• Grant number: 12/06033-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2012
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Patricia Jacqueline Thyssen
• Grantee: Mariana Prado Nassu
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Larval therapy involves the application of sterile maggots of necrobiontophagous flies alive(Diptera) obtained in laboratory on injuries, chronic wounds or infected. Its purpose is healing, from the removal of necrotic tissue and secretion by the insect, thereby facilitating the healing process. Despite the easy applicability, to ensure greater security and success in relation to this procedure two factors have to be achieved: the sterility of the larvae to be used and guarantee that this species use during their feeding process in the wound, only necrotic tissue. The present study aims to observe if the species Cochliomyia macellaria (Diptera: Calliphoridae), widely distributed in our Brazilian territory, could be useful for the application of larval therapy, taking into account their behavior and biology. We also intend to evaluate, using normal and diabetic rats with the presence of induced wounds as animal model, the quality and duration of treatment by the application of larvae compared to the usual mechanical debridement performed. In addition, it will be investigate aspects regarding the effect of larval density (number of larvae applied to the lesions/cm2) in order to reduce the treatment time in the patient.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MASIERO, FRANCIELE SOUZA; NASSU, MARIANA PRADO; SOARES, MAURO PEREIRA; THYSSEN, PATRICIA JACQUELINE. Histological patterns in healing chronic wounds using Cochliomyia macellaria (Diptera: Calliphoridae) larvae and other therapeutic measures. Parasitology Research, v. 114, n. 8, p. 2865-2872, AUG 2015. (12/06033-5)

AQUINO, M. F. K.; NASSU, M. P.; PEREIRA, D. I. B.; LEITE, D. S.; THYSSEN, P. J.. First Record of Larval Secretions of Cochliomyia macellaria (Fabricius, 1775) (Diptera: Calliphoridae) Inhibiting the Growth of Staphylococcus aureus and Pseudomonas aeruginosa. Neotropical Entomology, v. 46, n. 1, p. 125-129, FEB 2017. (12/06033-5)

DE MASIERO, FRANCIELE SOUZA; NASSU, MARIANA PRADO; SOARES, MAURO PEREIRA; THYSSEN, PATRICIA JACQUELINE. Histological patterns in healing chronic wounds using Cochliomyia macellaria (Diptera: Calliphoridae) larvae and other therapeutic measures. Parasitology Research, v. 114, n. 8, p. 8-pg., 2015-08-01. (12/06033-5)

NASSU, MARIANA PRADO; THYSSEN, PATRICIA JACQUELINE. Evaluation of larval density Cochliomyia macellaria F. (Diptera: Calliphoridae) for therapeutic use in the recovery of tegumentar injuries. Parasitology Research, v. 114, n. 9, p. 6-pg., 2015-09-01. (12/06033-5)

NASSU, MARIANA PRADO; THYSSEN, PATRICIA JACQUELINE. Evaluation of larval density Cochliomyia macellaria F. (Diptera: Calliphoridae) for therapeutic use in the recovery of tegumentar injuries. Parasitology Research, v. 114, n. 9, p. 3255-3260, SEP 2015. (12/06033-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NASSU, Mariana Prado. Use of Cochliomyia macellaria F. (Diptera: Calliphoridae) and evaluation of its larval density for therapeutic use in the recovery of tegumentar lesions. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.