COMPARATIVE STUDY OF CONTRACTILITY AND PASSIVE PROPERTIES OF THE MDX DIAPHRAGM IN DIFFERENT AGES

• Grant number: 12/04506-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2012
• End date: March 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Thais Borges Lessa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 13/06753-0 - Morpho-function analysis of the mdx's diaphragm with different ages, BE.EP.DR

Abstract

The Duchenne Muscular Dystrophy (DMD) is a genetic disease, X-linked characterized by mutation of the dystrophin gene, affecting children in the first two decades of life, whose main feature is the weakening and wasting of muscles. The main respiratory muscle affected is the diaphragm, which is responsible for 80% of the cause "of death." The use of mechanical forces that act on the body or intermittent pressure in the airways has improved the quality of life of patients, but does not prevent the progression of respiratory failure. Similarly the human DMD mdx model despite a milder phenotype, it has serious effects on the diaphragm, favoring the use of this model in respiratory implications. Given the need to establish a critical component to evaluate the effectiveness of experiments and expand the possibilities of pre-clinical trials aimed to establish, evaluate and compare the muscle strength and passive properties of the diaphragm muscle of mdx model mice and males with C57BL10 1, and 18 months of 6.12 idade.Será performed in all animals diaphragm test isometric tetanus test, elastic and viscous properties, with analysis by light microscopy and transmission electron. The variables are found statistically analyzed for multiple comparisons and variations will be considered heterogeneous.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HAKIM, CHADY H.; LESSA, THAIS B.; JENKINS, GREGORY J.; YANG, NORA N.; AMBROSIO, CARLOS E.; DUAN, DONGSHENG. An improved method for studying mouse diaphragm function. SCIENTIFIC REPORTS, v. 9, DEC 19 2019. (12/04506-3, 13/06753-0)

THAIS B. LESSA; DILAYLA K. ABREU; BRUNO M. BERTASSOLI; CARLOS E. AMBRÓSIO. Arquitetura comparativa dos pulmões de camundongos normais e afetados pela Distrofia Muscular de Duchenne. Pesquisa Veterinária Brasileira, v. 35, p. 56-60, 2015-12-00. (12/04506-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LESSA, Thais Borges. Comparative study of the active and passive properties in mdx/utrn +/- and mdx with different ages. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.