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Expression, Purification, Cristalization and Functional Assays of the enzime Beta-galactosidase from the Arqueobacteria Haloterrigena turkmenica

Grant number: 12/08402-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2012
Effective date (End): June 30, 2013
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal researcher:Igor Polikarpov
Grantee:Fernando Peron Magrini
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis, AP.BIOEN.TEM

Abstract

The cell wall is based on lignocellulose, including three primordial componentes: the cellulose, hemicellulose and lignine. Enzimes of the glycosil hydrolasis kind acts on this types of bonds between cell wall components. By this reason, those enzymes became an important target to cell wall hydrolysis. The beta-galactosidase acts on bonds ²(1’4) between sugars of lactose. This enzyme is the major producer of galactooligosaccharides, a important type of prebiotic used in functional food, such ass yogurts. Being the main source of galactooligosaccharides, the beta-galactosidase has grown in interest at industry and biotechnology. Our goal is to express, purify, crystallize and perform biochemicals and cinetics assays in beta-galactosidase from the archaobacteria Haloterrigena turkmenica, which grows at extreme conditions of salinity and temperature. The results of this study will help to comprehend the molecular mechanisms of these enzymes.