Molecular identification of virulent genes from LEE region and not LEE from EPEC strains isolated from animals and humans

• Grant number: 12/09306-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2012
• End date: June 30, 2013
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Fernando Antonio de Avila
• Grantee: Samara Beretta Figueiredo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Enteropathogenic Escherichia Coli (EPEC) is an E. Coli category with public health interest , once responsible for diarrhea, mostly in children from developing countries. This project goal determinate the presence of genes related to LEE Region and not LEE from EPEC in isolated originating from animals (buffalo, pigs and ovines) and humans. Will be studied also Escherichia Coli culture, isolated from unidentified human feces in MacConkey agar plates lent by USP hospital clinical laboratory in Ribeirão Preto - SP. The strains originating from animals and humans will be tested through PCR selection for eae genes identification. After selection will be better categorized by PCR as present espB (E2348/69), espB (EO26), espB (EDL933), espD, espD (EDL933), espF, pEAF, tir±, tir², tir³, nleA, nleB, nleE, nleF e nleH1-2 genes. EPEC isolated will be undergone by susceptibility and antimicrobial tests and serological tests to define sorogroups. Beyond that gene detection statiscally associated to diarrhea in EPEC can show if isolated belonging to studied animals present zoonotic potential.(AU)