Padronization of histopathological rat periodontum analysis in orthodontic research

• Grant number: 12/10557-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Marcelo Macedo Crivelini
• Grantee: Verônica Honda Gomes de Oliveira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Regarding published orthodontic research that assesses microscopically animals' periodontium, an issue that must be improved is to standardize both methodology and data collection protocols, once several of them are limited only by histopathological descriptions. The aim of this study is to establish the macroscopic metric values and histometric protocols to standardize histopathological observations, especially when are involved parasagittal mesiobuccal and distobuccal superior first molar roots of young rats under dental induced movement. The present study will assess 20 hemimaxilas of Wistar rats, in which macroscopic sections will follow a pre-established protocol. Histological sections of periodontium will be assessed by history to define the tolerance average values and consider them suitable for analysis. Therefore several histological cuts among the different groups will be ensured regarding the same histological cuts level, with similar structures and histometric values.(AU)