Hormone replacement therapy in the ventral lobe of prostate of UChB rat: effects on estrogen receptors (er alpha e er beta) and the signaling pathway of TGF-beta 1

• Grant number: 12/00917-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 01, 2012
• End date: October 31, 2012
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Francisco Eduardo Martinez
• Grantee: Leonardo de Oliveira Mendes
• Supervisor: Jan-Åke Gustafsson
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Houston (UH), United States

Abstract

Excessive alcohol consumption and its consequences arising from their use have reached alarming proportions around the world. One of the effects of ethanol is a decrease of testosterone concentration that alter the androgen-estrogen ratio and may have a direct effect on the prostate gland, hormone-dependent. The estrogen receptor ± and ² are important in prostate homeostasis and its dysregulation is associated with pathologies.Recent research has focused on the hormonal imbalance as a factor triggering inflammatory processes. In inflammatory foci, occurs the local release of cytokines (interferon - IFN, interleukin - IL, colony stimulating factor - CSF, tumor necrosis factor - TNF ± and ² and transforming growth factor - TGF ²). TGF-²1 regulates cell proliferation, differentiation and apoptosis and their dysregulation is associated with prostatic hyperplasia and cancer. This study will assess the effects of ethanol and hormone replacement with testosterone on the localization and quantification of estrogen receptors (± and ²) and proteins of the signaling pathway of TGF-²1 (TGF-²1, Smad 2 / 3 and a snail) in the ventral lobe of prostate of UChB rat. The methods will be employed at the Centre for Nuclear Receptor and Cell Signaling of the University of Houston, under the supervision of Prof. Dr. Jan-Ake Gustafsson. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, LEONARDO O.; SCARANO, WELLERSON R.; ROCHEL-MAIA, SABRINA S.; FIORUCI-FONTANELI, BEATRIZ A.; CHUFFA, LUIZ G. A.; ANSELMO-FRANCI, JANETE A.; MARTINEZ, FRANCISCO E.. Androgen therapy reverses injuries caused by ethanol consumption in the prostate: Testosterone as a possible target to ethanol-related disorders. Life Sciences, v. 120, p. 22-30, JAN 1 2015. (11/05936-9, 12/00917-9)

MENDES, LEONARDO O.; CASTILHO, ANTHONY C. S.; PINHO, CRISTIANE F.; GONCALVEZ, BIANCA F.; RAZZA, EDUARDO M.; CHUFFA, LUIZ GUSTAVO A.; ANSELMO-FRANCI, JANETE A.; SCARANO, WELLERSON R.; MARTINEZ, FRANCISCO E.. Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats. Cell Biology International, v. 42, n. 9, p. 1200-1211, SEP 2018. (11/05936-9, 12/00917-9)

LIMA, BRUNA JARDIM PEREIRA; DE OLIVEIRA, GABRIEL RODRIGUES LEAL; SOUSA, THAINA CAVALLERI; DE AQUINO, ARIANA MUSA; HINOKUMA, KARIANNE DELALIBERA; RICARDO, MARIA LUIZA SILVA; SCARANO, WELLERSON RODRIGO; CASTILHO, ANTHONY CESAR DE SOUZA; PACAGNELLI, FRANCIS LOPES; MARTINEZ, FRANCISCO EDUARDO; et al. Fractal analysis is a useful tool for evaluating prostate tissue remodeling caused by ethanol consumption and androgen therapy. ANIMAL REPRODUCTION, v. 20, n. 3, p. 10-pg., 2023-01-01. (12/00917-9)