Biodiversity of Selenastraceae (freshwater Chlorococcaceae microalgae): morphological characteristics and sequencing of 18S rDNA as a traditional taxonomic basis for obtaining the DNA barcode

• Grant number: 12/19520-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Botany - Plant Taxonomy
• Principal Investigator: Armando Augusto Henriques Vieira
• Grantee: Thaís Garcia da Silva
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 11/50054-4 - Biodiversity of freshwater microalgae: germplasm bank and obtaining of molecular markers of cryopreserved species, AP.BTA.TEM
• Associated scholarship(s): 13/17457-3 - Traditional taxonomy and analysis of the 18S rDNA, ITS2 and rbcL sequences of species of the freshwater microalgae family Selenastraceae, BE.EP.DD

Abstract

This project proposes the use of traditional practices of taxonomy and analysis of the sequencing of the 18S rDNA of species of freshwater microalgae family Selenastraceae. The main objective is to provide a basis for taxonomic identification of these organisms, whose biodiversity is currently scattered in "complex" Ankistrodesmaceae / Selenastraceae / Chlorellaceae / Oocystaceae, a taxonomically problematic group. For species level resolution, obtaining DNA barcode is a powerful tool, but it does not obviate the practices of traditional taxonomy: morphology, chemotaxonomy, and other collateral information (Hajibabaei et al. 2007). Organisms must be preliminarily entered into a reliable taxonomic system even if, in some groups, it may not be enough to differentiate the species without errors (as is the case of microalgae with their high morphological convergence rates, high incidence of polymorphism and cryptic species). In these cases, the DNA barcode associated with traditional practices of the taxonomy may be the definitive factor in the proper identification of the species. A relatively sound taxonomic base, based on morphological characters, 18S rDNA phylogenetic analysis and specific biochemical profiles is a great starting point for the provision of "vouchers" for obtaining DNA barcodes. In Chloroccocaceae, the morphological features, although subjective, are extremely useful and necessary as a starting point, and in many cases might be determinants for differentiation (Fawley et al. 2005). However, morphological differences between the species are often only detectable through the crops, and therefore the species employed in this study will be grown and sampled at various stages of specific growth. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, THAIS GARCIA; BOCK, CHRISTINA; SANT'ANNA, CELIA LEITE; BAGATINI, INESSA LACATIVA; WODNIOK, SABINA; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Selenastraceae (Sphaeropleales, Chlorophyceae): rbcL, 18S rDNA and ITS-2 secondary structure enlightens traditional taxonomy, with description of two new genera, Messastrum gen. nov. and Curvastrum gen. nov.. Fottea, v. 17, n. 1, p. 1-19, 2017. (12/19520-1, 13/17457-3, 11/50054-4, 13/18083-0)

GARCIA, T. S.; BOCK, CHRISTINA; BAGATINI, INESSA LACATIVA; ARCHANJO, NAIARA CAROLINA PEREIRA; SANT'ANNA, CELIA LEITE; VIEIRA, ARMANDO AUGUSTO HENRIQUES. Hidden diversity in Selenastraceae (Sphaeropleales, Chlorophyceae): Molecular phylogeny revealed a nannoplanktonic species, with the description of Viridiparva madeirensis gen. et sp. nov.. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 68, p. 9-pg., 2022-11-11. (11/50054-4, 13/17457-3, 12/19520-1, 19/03177-5, 13/18083-0, 16/07089-5)

BAGATINI, INESSA LACATIVA; MECCHERI, FABRICIO SEBASTIANI; PAULSEN, BERIT SMESTED; DA SILVA, THAIS GARCIA; BARSETT, HILDE; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Diversity of monosaccharides and glycosidic linkages on extracellular polysaccharides of the microalga Ankistrodesmus (Chlorophyceae). PHYCOLOGIA, v. N/A, p. 12-pg., 2022-11-14. (13/18083-0, 19/03177-5, 13/17457-3, 12/19520-1, 12/00221-4, 11/50054-4)

DA SILVA, THAIS GARCIA; STENCLOVA, LENKA; PEREIRA ARCHANJO, NAIARA CAROLINA; BAGATINI, INESSA LACATIVA. Revised phylogenetic position of Nephrocytium Nageli (Sphaeropleales, Chlorophyceae), with the description of Nephrocytiaceae fam. nov. and Nephrocytium vieirae sp. nov.. Taxon, v. 70, n. 5, AUG 2021. (19/03177-5, 11/50054-4, 13/18083-0, 16/07089-5, 13/17457-3, 12/19520-1)

MORI, CILENE CRISTINA; BAGATINI, INESSA LACATIVA; DA SILVA, THAIS GARCIA; PARRISH, CHRISTOPHER CHARLES; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Use of fatty acids in the chemotaxonomy of the family Selenastraceae (Sphaeropleales, Chlorophyceae). Phytochemistry, v. 151, p. 9-16, JUL 2018. (13/03979-8, 11/50054-4, 12/19520-1, 13/18083-0)