Neonatal maternal separation impacts on gastric mucosa development in pups and adult rats: Role of corticosterone and GR

• Grant number: 12/10948-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2013
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Patrícia Gama
• Grantee: Daniela Ogias
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Gastrointestinal growth is a dynamic process coordinated by many factors: diet, hormones, growth factors and genetic program. Any disturbance during early life induces changes in growth which can last until adulthood. Neonatal maternal separation includes maternal care and food deprivation. We know that fasting increases plasma CORT and CBG binding, but decreases GR intracellular traffic in pups. CORT can have direct or indirect roles in the gastric mucosa growth and development. In the stomach, cell differentiation is a gradual process which is completed by the end of the third postnatal week, in parallel with plasma CORT increase. It is not clear how corticosterone and maternal separation modify gastric epithelium growth and differentiation. So by using the rat neonatal maternal separation model, our aims are :1) evaluate effects on CORT availability, 2) to evaluate the gastric mucosa growth, 3) to analyze the impacts in development and cell differentiation in pups, and the consequences to the adult stomach. For this purpose we will measure the plasma CORT levels by RIA and its availability through CBG binding, study GR distribution and binding to GRE´s. We will also investigate markers of cell differentiation through the expression of GR target genes and we will evaluate the proteins that are encoded by these genes by Western blot and immunohistochemistry.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TELES SILVA, MELISSA; MESQUITA DA SILVA, KETHLEEN; CAMPOS RATTES, ISADORA; MARIA AGOSTINI ZONTA, GIZELA; VASQUES DA COSTA, ALINE; GALVAO FIGUEREDO COSTA, RAQUEL; KAREN CORDEIRO NOGUEIRA, LUDIMILA; OGIAS, DANIELA; GAMA, PATRICIA. Immediate and Late Effects of Early Weaning on Rat Gastric Cell Differentiation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 1, JAN 1 2020. (11/17415-3, 12/10948-9, 14/21449-9, 16/21944-5, 18/24972-5, 18/07782-8)

OGIAS, DANIELA; RATTES, ISADORA C.; HOSOYA, LARISSA Y. M.; ZULIAN, JULIANA G.; YAN, CHAO YUN IRENE; GAMA, PATRICIA. Neonatal- maternal separation primes zymogenic cells in the rat gastric mucosa through glucocorticoid receptor activity. SCIENTIFIC REPORTS, v. 8, JUN 29 2018. (12/10948-9, 11/17415-3, 14/21449-9, 10/03076-0, 13/18459-0)

OGIAS, D.; RATTES, I.; ZULIAN, J. G.; RIGONATTI, C. A.; GAMA, P.. Can gastric cell differentiation be reprogrammed by early life stress?. MOLECULAR BIOLOGY OF THE CELL, v. 26, p. 2-pg., 2015-01-01. (14/21449-9, 11/17415-3, 12/10948-9)