The occurrence of Cryptosporidium spp. in buffalo steers in the city of Alambari, São Paulo

• Grant number: 12/14882-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2013
• End date: March 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Katia Denise Saraiva Bresciani
• Grantee: Débora Barbosa Bruno
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This research project aims to evaluate the occurrence of the Cryptosporidium in buffalo steers up to six months old in the city of Alambari, São Paulo. Fecal samples of an amount of 220 animals will be examined through the Kinyoun technique, to verify the elimination of oocysts and through the immunosorbent assay (ELISA), using the "Cryptosporidium Test" kit by TechLab to the antigens detection. The samples that present to be positive to the microscopy or to the ELISA test will be molecularly characterized by a polymerase chain reaction to 18S from rRNA subunit fragment amplification and amplified fragments sequencing. The statistical analysis will consist of a Chi-Square test to verify the association of the referred enteroparasite relative to the gender, race, and age of the analyzed animals.(AU)