Analysis of inflammasome in melanoma: correlation between tumoral cells and immune response mediated by dendritic cells

• Grant number: 12/18448-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2013
• End date: April 30, 2017
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Telma Miyuki Oshiro Sumida
• Grantee: Wanessa Cardoso da Silva
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

It has been demonstrated that the progression of melanoma is closely related to an inflammatory microenvironment. In this sense, IL-1ß is an important pro-inflammatory mediator capable of coordinating the expression of other cytokines and chemokines, and mediate the recruitment of cells to the site of injury. In turn, the maturation of IL-1ß as well as its secretion is controlled by a cytoplasmic molecular complex known as inflammasome. The inflammasomes are expressed mainly in cells of innate immunity and dendritic cells (DCs). DCs have a major role in the driving the specific immune response that depends on the characteristics of DCs and the microenvironment of occurrence of antigen presentation. In this context, this project aims to investigate the impact of inflammasome on the immune response against melanoma through the analysis of its activation in monocyte-derived DC and in biopsies from melanoma patients. The genetic susceptibility will also be investigated by analyzing polymorphisms in genes of inflammasome in a cohort of melanoma patients. We expected that the results obtained from this project can provide information regarding the pathogenetic mechanisms involved in melanoma development and in the immune response against this type of cancer, as well as discover novel targets for future therapeutic approaches. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, WANESSA CARDOSO; OSHIRO, TELMA MIYUKI; DE SA, DANIEL COELHO; FRANCO, DILCILEA D. G. S.; NETO, CYRO FESTA; PONTILLO, ALESSANDRA. Genotyping and differential expression analysis of inflammasome genes in sporadic malignant melanoma reveal novel contribution of CARD8, IL1B and IL18 in melanoma susceptibility and progression. CANCER GENETICS, v. 209, n. 10, p. 474-480, OCT 2016. (13/06142-1, 12/18448-5, 12/09824-3)

DA SILVA, WANESSA CARDOSO; OSHIRO, TELMA M.; DE SA, DANIEL COELHO; FRANCO, DILCILEA D. G. S.; NETO, CYRO FESTA; PONTILLO, ALESSANDRA. Data on inflammasome gene polymorphisms of patients with sporadic malignant melanoma in a Brazilian cohort. DATA IN BRIEF, v. 10, p. 33-37, FEB 2017. (13/06142-1, 12/18448-5, 12/09824-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Wanessa Cardoso da. Contribution of inflammasome genetics in the predisposition to develop sporadic malignant melanoma. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.