Non-invasive methodologies with enhanced sensitivity for diagnosing human Bartonella spp. infection

• Grant number: 12/22340-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2013
• End date: April 30, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Paulo Eduardo Neves Ferreira Velho
• Grantee: Marilene Neves da Silva
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Bartonellas are bacteria worldwide distribution, re-emerging and neglected. The bartonella infections are potentially fatal, especially in immunocompromised patients. These bacteria invade endothelial cells and there is no gold standard diagnostic method, because false negative results are common even using serological and molecular techniques. The objective of this study is to find new non-invasive methods and more sensitive to diagnose infection by Bartonella spp .. Balb / c mice are infected by known concentration of the agent and uninfected animals serve as a control. After four days of experimental infection blood samples will be collected for isolation of endothelial cells, inflammatory cytokine analysis and PCR. Animal skin tissue culture will be made and these will be analyzed by optical microscopy and transmission electron. As a result of the research, expected to generate new diagnostic tests to detect infection with Bartonella spp., A new model for studying biofilm formation by Bartonella spp. and their resistance to antibiotics.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, MARILENE NEVES; VIEIRA-DAMIANI, GISLAINE; ERICSON, MARNA ELISE; GUPTA, KALPNA; DE ALMEIDA, AMANDA ROBERTA; DRUMMOND, MARINA ROVANI; BENETTI SOARES, TANIA CRISTINA; LANIA, BRUNO GROSSELLI; GILIOLI, ROVILSON; NEVES FERREIRA VELHO, PAULO EDUARDO. Acute and Late Bartonella henselae Murine Model Infection. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 17, n. 3, p. 206-208, MAR 2017. (12/22340-5)

DA SILVA, MARILENE NEVES; VIEIRA-DAMIANI, GISLAINE; ERICSON, MARNA ELISE; GUPTA, KALPNA; GILIOLI, ROVILSON; DE ALMEIDA, AMANDA ROBERTA; DRUMMOND, MARINA ROVANI; LANIA, BRUNO GROSSELLI; LINS, KARINA DE ALMEIDA; BENETTI SOARES, TANIA CRISTINA; et al. Bartonella henselae transmission by blood transfusion in mice. Transfusion, v. 56, n. 6, 2, SI, p. 1556-1559, JUN 2016. (12/22340-5)

DA SILVA, MARILENE NEVES; VIEIRA-DAMIANI, GISLAINE; ERICSON, MARNA ELISE; GUPTA, KALPNA; GILIOLI, ROVILSON; DE ALMEIDA, AMANDA ROBERTA; DRUMMOND, MARINA ROVANI; LANIA, BRUNO GROSSELLI; LINS, KARINA DE ALMEIDA; BENETTI SOARES, TANIA CRISTINA; et al. Bartonella henselae transmission by blood transfusion in mice. Transfusion, v. 56, n. 6, p. 4-pg., 2016-06-01. (12/22340-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Marilene Neves da. Translational research about infection by Bartonella henselae and its transfusion transmission. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.