Structural, biochemical and phenotypic studies of CHASE receptors associated with bacterial domains GGDEF / EAL

• Grant number: 12/25276-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2013
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Marcos Vicente de Albuquerque Salles Navarro
• Grantee: Everton Edesio Dinis Silva
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 09/13238-0 - Structural and functional studies of proteins involved in c-di-GMP-mediated signalling pathways , AP.JP

Abstract

The pathways of biosynthesis and degradation of c-di-GMP are widely used by bacteria to coordinate the cellular machinery in the assimilation of phenotypes according to the environmental conditions. Some of them, such as virulence, biofilm formation and cell cycle control often cause obstacles in everyday human. A key regulator of such biological phenomena is a ubiquitous second messenger molecule, c-di-GMP. The regulation of protein catalytic domains (GGDEF and EAL) responsible for c-di-GMP biosynthesis are often linked to other domains accessories. The structural knowledge of such domains could provide an alternative molecular target to fight bacterial undesirable phenotypes. Among these accessory domains, an interesting class of periplasmic sensor recently identified (CHASE) is found in several bacterial transmembrane proteins containing GGDEF and EAL domains. Despite CHASE domain are widely studied in plants and protozoa, no information about its relationship with c-di-GMP signaling pathways is known. Thus, this study aims to unravel the mechanism of action of such proteins through structural, biochemical and phenotypic studies. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Everton Edesio Dinis. Regulation studies of signalling transducers PA0847 and HsbD from Pseudomonas aeruginosa and GGDEF and EAL systems in tandem. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.