Identification of the microbiota of facial prostheses and laboratory evaluation of the effectiveness of different hygiene solutions.

• Grant number: 12/24853-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2013
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Cláudia Helena Lovato da Silva
• Grantee: Juliana Barchelli Pinheiro
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of this study is to identify the microbial biofilm present in prosthetics and facial injuries by method of DNA hybridization checkerboard and also assess laboratory antimicrobial action of three methods of cleaning on microorganisms identified in specimens made with a silicone experimental compared to a commonly employed in the art (MDX 4-4210 - baseline), after pigmentation. The specimens will be randomly divided into four groups of pigmentation: 1. Pigmented powder makeup 2. Pigmented with opacifier 3. Pigmented powder makeup associated with opacifier. As a control, specimens will be used colorless. After this first step, will be made circular 320 specimens (15 mm diameter x 3 mm thick) from an array of prefabricated glass plex, and 160 with each material, with the aim of evaluating the influence of laboratory methods care about the color change, Shore A hardness, surface roughness and water absorption of silicones. At the end, the surface of the specimens will be evaluated by scanning electron microscopy (SEM) to evaluate the methods of sanitizing influenced the surface of the specimens. After obtaining the data, the data will be analyzed and analyzed statistically relevant.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINHEIRO, JULIANA BARCHELLI; VOMERO, MARINA PERIS; DO NASCIMENTO, CASSIO; WATANABE, EVANDRO; OLIVEIRA PARANHOS, HELENA DE FREITAS; COTO, NEIDE PENA; DIAS, REINALDO BRITO; DE OLIVEIRA, VIVIANE CASSIA; SILVA-LOVATO, CLAUDIA HELENA. Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols. BIOFOULING, v. 34, n. 1, p. 15-25, 2018. (12/24853-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PINHEIRO, Juliana Barchelli. Identification of the microbiota of maxillofacial prostheses and adjacent tissues and evaluation of the antimicrobial effectiveness of different hygiene protocols and their effects on properties of two silicones. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.