MICROBIOLOGICAL EVALUATION OF FLUID PIPE SONICATION ENDOTRACHEAL REMOVED FOR PEDIATRIC PATIENTS IN THE INTENSIVE CARE UNIT AND COMPARISON WITH THE CULTURE OF SECRETION RESULATADOS TRACHEAL

• Grant number: 13/00129-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2013
• End date: April 30, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Mauro José Costa Salles
• Grantee: Gabriel Fialkovitz da Costa Leite
• Host Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP). São Paulo , SP, Brazil

Abstract

Nosocomial pneumonia, especially when associated with mechanical ventilation is one of the most common causes of death from infection in patients in the ICU. In pediatric patients under intensive care, this type of infection occurs in about 2.3 cases per 1000 hours of ventilation.The diagnosis of ventilator-associated pneumonia (VAP) is based on radiological criteria associated with clinical and microbiological profile based on samples obtained commonly by endotracheal suctioning. The secretion culture obtained by this methoddespite being the most widely used, has data with high sensitivity and specificity variations, subjecting the occurrence of false positives and false negatives.Aiming to improve these data, the culture of the fluid of sonication of the endotracheal tube, the method by which the application of ultrasound detaches the layer of biofilm from the surface of material, has emerged as a new diagnostic approach. The objective of this study is to validate the culture of the fluid of sonication of endotracheal tube as complementary diagnosis in patients with VAP, and comparatively evaluate the quantitative culture of tracheal secretions with sonication fluid from the biofilm attached to the endotracheal tube in the same patients diagnosed with VAP.The study is a prospective pilot of microbiological analysis and comparison between the results obtained by culture form the fluid of sonication of endotracheal tubes and cultures derived from tracheal secretions collected during the intubation, and when withdrawal of the tube.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

FERREIRA, THIAGO DE OLIVEIRA; KOTO, RAFAEL YOSHIO; DA COSTA LEITE, GABRIEL FIALKOVITZ; KLAUTAU, GISELLE BURLAMAQUI; NIGRO, STANLEY; DA SILVA, CELY BARRETO; IDALGO DA FONSECA SOUZA, ANA PAULA; MIMICA, MARCELO JENNE; CESAR, REGINA GRIGOLLI; COSTA SALLES, MAURO JOSE. Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients. Brazilian Journal of Infectious Diseases, v. 20, n. 5, p. 468-475, SEP-OCT 2016. (13/00129-3)