Natural products in wax moth control and its effects over bees Apis melífera L

• Grant number: 13/07827-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2013
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Ricardo de Oliveira Orsi
• Grantee: Daniele Maria Telles
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The beekeeping is responsible for important roles in the country, such as on the environment and social life. However, as any other crop, is susceptible to attack by many plagues and diseases. A plague that can cause serious damage to beekeeping is called wax moth (Galleria Mellonella), an insect from the Lepdoptera Order, whose destroys the honeycombs wax, consuming the pollen and bee brood, leading the hive to extinction. The fight can be carried out through chemical and/or physical methods, which might become undesirable because they may contaminate the honey and other bee products or to be extremely expensive. An alternative would be the biological control, made with natural products that does not expose the bees and their products to all the contamination risks. With everything that is being said the goals of this work will be analyze the NIM Oil (Azadirachta indica), nicotine (Nicotiana tabacum), Pepper Extract (Piper sp) and the efficiency of Eucalyptus Essential Oil (Eucalyptus sp) in Moth wax (Galleria Mallonella) control, and its direct effects over a bee colony development. To the in vitro tests, grown bees (put in cages and sprayed with 2,0 mL of all the different mentioned products) and moth larvae (placed in petri dishes containing moistened filter paper with 1,5 mL of all the different products) will be submitted to different amounts of the products mixed with distilled water (0,0%; 0,5%; 1,0%; 2,5%; 5,0%; 10,0%; 20,0%; 40,0%; 80,0% e 100,0%). Mortality observations will be done every 15 minutes, for 4 hours. The tests will be performed 5 times. To study the products effects in the hive development population, its central part, containing the brood area will be selected. In it, 2,0 mL of the solution of the products that showed the best results during the in vitro tests will be besprent, the counts will be done for 35 days, with weekly evaluations. The results will be compared by ANOVA, followed by the Tukey's test to verify the differences among the averages. It will be considered statistically different when p<0,05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TELLES, D. M.; MARTINELI, G. M.; SCALOPPI, M. F.; FERREIRA DA LUZ, M. P.; KADRI, S. M.; ORSI, R. O.. Natural Products can Efficiently Control the Greater Wax Moth (Lepidoptera: Pyralidae), but are Harmless to Honey Bees. Sociobiology, v. 67, n. 1, p. 89-93, MAR 2020. (13/07827-8)