The Effects of anatoxin-a and cylindrospermopsin at different nitrogen concentrations and photon irradiances on the physiology of Microcystis aeruginosa (Cyanobacteria) and Scenedesmus acuminatus (Chlolrophyta)

• Grant number: 13/11306-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2013
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Ecology
• Principal Investigator: Maria Do Carmo Bittencourt de Oliveira
• Grantee: Mathias Ahii Chia
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 14/26898-6 - Unravelling some physiological and molecular processes involved in the allelopathic interactions between toxic and non-toxic phytoplankton, BE.EP.PD

Abstract

Interactions between cyanobacteria and their environment result in the production of secondary metabolites that are classified as being allelochemicals and toxins. These are geared towards ensuring their cell proliferation in the aquatic system and out competing neighboring micro- and macro-plants and cyanobacteria for available nutrients and space. Among the cyanotoxins produced by species of cyanobacteria, cylindrospermopsin (CYN) and anatoxin-a (ATX) have been the least studied with regards to their effects on other algae under different biotic and abiotic environmental conditions. This study will investigate the effects of CYN and ATX on the growth, biomass production and antioxidant enzyme activities of Microcystis aeruginosa (BCCUSP232) and Scenedesmus acuminatus (UFSCar036), and microcystins (MCs) production by M. aeruginosa under different nitrogen (0.04 to 9 mM NaNO3) concentrations and photon irradiances (10 and 40 µmol photons m-2 s-1). Four different experiments will be carried out. In the first and second experiments, the effects of pure ATX at environmentally relevant concentrations on M. aeruginosa and S. acuminatus under varying nitrogen concentrations and photon irradiances will be studied. The third and fourth experiments will determine how CYN influences the growth and physiology of M. aeruginosa and S. acuminatus under different N concentrations and photon irradiances. The results of this project will enhance our current understanding of how cyanotoxins influence cyanobacterial and microalgal physiology under varying environmental conditions using realistic and environmentally relevant concentrations of the toxins, as well as the establishment of the possible influence of ATX and CYN on MCs production by M. aeruginosa.

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KADIRI, MEDINA O.; ISAGBA, SOLOMON; OGBEBOR, JEFFREY U.; OMORUYI, OSASERE A.; UNUSIOTAME-OWOLAGBA, TIMOTHY E.; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CHIA, MATHIAS AHII. The presence of microcystins in the coastal waters of Nigeria, from the Bights of Bonny and Benin, Gulf of Guinea. Environmental Science and Pollution Research, v. 27, n. 28, SI, JUN 2020. (14/26898-6, 13/15296-2, 13/11306-3, 14/01913-2)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; DE TOLEDO ARRUDA-NETO, JOAO DIAS; TORNISIELO, VALDEMAR LUIZ; VILCA, FRANZ ZIRENA; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Microcystin-LR bioaccumulation and depuration kinetics in lettuce and arugula: Human health risk assessment. Science of The Total Environment, v. 566, p. 1379-1386, OCT 1 2016. (13/11306-3, 14/01934-0, 15/17397-6)

CHIA, MATHIAS A.; JANKOWIAK, JENNIFER G.; KRAMER, BENJAMIN J.; GOLESKI, JENNIFER A.; HUANG, I-SHUO; ZIMBA, PAUL V.; BITTENCOURT-OLIVEIRA, MARIA DO CARMO; GOBLER, CHRISTOPHER J.. Succession and toxicity of Microcystis and Anabaena (Dolichospermum) blooms are controlled by nutrient-dependent allelopathic interactions. HARMFUL ALGAE, v. 74, p. 67-77, APR 2018. (13/11306-3, 14/26898-6)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Growth and antioxidant response of Microcystis aeruginosa (Cyanobacteria) exposed to anatoxin-a. HARMFUL ALGAE, v. 49, p. 135-146, NOV 2015. (13/11306-3, 14/26898-6, 14/01934-0)

LORENZI, ADRIANA STURION; CHIA, MATHIAS AHII; PICCIN-SANTOS, VIVIANE; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: a case study of northeastern Brazilian reservoirs. LIMNETICA, v. 34, n. 2, p. 269-281, DEC 2015. (13/11306-3, 14/01913-2)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Potential human health risk assessment of cylindrospermopsin accumulation and depuration in lettuce and arugula. HARMFUL ALGAE, v. 68, p. 217-223, SEP 2017. (13/11306-3, 14/01934-0, 15/17397-6)

LORENZI, ADRIANA STURION; CHIA, MATHIAS AHII; CARDOSO LOPES, FABYANO ALVARES; SILVA, GENIVALDO GUEIROS Z.; EDWARDS, ROBERT A.; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology. JOURNAL OF MICROBIOLOGY, v. 57, n. 6, p. 450-460, JUN 2019. (13/11306-3, 14/01913-2, 13/15296-2)

BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; DE TOLEDO ARRUDA-NETO, JOAO DIAS; DE OLIVEIRA, ENIO TIAGO; DOS SANTOS, FLAVIO. Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 128, p. 83-90, JUN 2016. (13/11306-3, 14/01934-0)

CHIA, MATHIAS AHII; BITTENCOURT-OLIVEIRA, MARIA DO CARM. Allelopathic interactions between phytoplankton species alter toxin production, oxidative response, and nitrogen fixation. Hydrobiologia, v. 848, n. 19, JUL 2021. (14/26898-6, 13/11306-3)

CHIA, MATHIAS AHII; KRAMER, BENJAMIN J.; JANKOWIAK, JENNIFER G.; BITTENCOURT-OLIVEIRA, MARIA DO CARMO; GOBLER, CHRISTOPHER J.. The Individual and Combined Effects of the Cyanotoxins, Anatoxin-a and Microcystin-LR, on the Growth, Toxin Production, and Nitrogen Fixation of Prokaryotic and Eukaryotic Algae. TOXINS, v. 11, n. 1, JAN 2019. (13/11306-3, 14/26898-6)

LORENZI, ADRIANA STURION; CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Cyanotoxin contamination of semiarid drinking water supply reservoirs. ENVIRONMENTAL EARTH SCIENCES, v. 77, n. 16, AUG 2018. (13/11306-3, 14/01913-2, 15/17397-6)

BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CHIA, MATHIAS AHII; BEZERRA DE OLIVEIRA, HELTON SORIANO; CORDEIRO ARAUJO, MICHELINE KEZIA; REIS MOLICA, RENATO JOSE; SANTOS DIAS, CARLOS TADEU. Allelopathic interactions between microcystin-producing and non-microcystin-producing cyanobacteria and green microalgae: implications for microcystins production. JOURNAL OF APPLIED PHYCOLOGY, v. 27, n. 1, p. 275-284, FEB 2015. (13/11306-3, 11/50840-0)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Cylindrospermopsin induced changes in growth, toxin production and antioxidant response of Acutodesmus acuminatus and Microcystis aeruginosa under differing light and nitrogen conditions. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 142, p. 189-199, AUG 2017. (13/11306-3, 13/15296-2, 15/17397-6, 14/26898-6, 14/01934-0)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; HEREMAN, TALITA CAROLINE; SASAKI, FABIANA FUMI; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Selective membrane permeability and peroxidase activity response of lettuce and arugula irrigated with cyanobacterial-contaminated water. ENVIRONMENTAL EARTH SCIENCES, v. 74, n. 2, p. 1547-1553, JUL 2015. (13/11306-3, 14/01934-0)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Does anatoxin-a influence the physiology of Microcystis aeruginosa and Acutodesmus acuminatus under different light and nitrogen conditions?. Environmental Science and Pollution Research, v. 23, n. 22, p. 23092-23102, NOV 2016. (13/11306-3, 14/26898-6, 14/01934-0, 13/15296-2)

BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CHIA, MATHIAS AHII; CAMARGO-SANTOS, DANILO; DIAS, CARLOS T. S.. The effect of saxitoxin and non-saxitoxin extracts of Cylindrospermopsis raciborskii (Cyanobacteria) on cyanobacteria and green microalgae. JOURNAL OF APPLIED PHYCOLOGY, v. 28, n. 1, p. 241-250, FEB 2016. (13/11306-3, 11/02957-5, 11/50840-0)

KADIRI, MEDINA O.; ISAGBA, SOLOMON; OGBEBOR, JEFFREY U.; OMORUYI, OSASERE A.; UNUSIOTAME-OWOLAGBA, TIMOTHY E.; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CHIA, MATHIAS AHII. The presence of microcystins in the coastal waters of Nigeria, from the Bights of Bonny and Benin, Gulf of Guinea. Environmental Science and Pollution Research, v. 27, n. 28, p. 10-pg., 2020-06-26. (13/15296-2, 13/11306-3, 14/01913-2, 14/26898-6)