Identification and study of the relationship between tricodinídeos and larvae of Nile tilapia

• Grant number: 13/20067-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2013
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Fabiana Pilarski
• Grantee: Lindomar de Oliveira Alves
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The larvae hatchery is a decisive step to aquatic organisms, it will be responsible for ensuring health for fingerling and fattening stages. Currently, this stage production is a source disseminator of parasites due to lack of health programs and biosecurity in aquaculture farms. Ectoparasites can cause yield losses due to injuries, often irreversible and facilitate the installation of opportunistic agents that cause the host death.The trichodina are today the main ectoparasites of Nile tilapia in Brazil and around the world, and have been often found from the larval stage to fish in the finishing phase. Thus, we propose, in this study, parasitological analysis of Nile tilapia larvae from three different hatcheries in the state of São Paulo, with a history of massive infestations by tricodinídeos. Ten larvae of each fish will be used to identify the species of existing tricodinídeos, which will be euthanized by section of the medulla and compressed between slide and cover slip. Slides containing the parasites are impregnated with silver nitrate and stained with 2% Giemsa. The morphology of the parasites will be performed using the software Image Pro Plus ®, while the morphology is described from schematic drawings vectorized produced with CorelDRAW ® X6. The study of the relationship between tricodinídeos and larvae of tilapia will be performed by light microscopy (histology) and scanning electron microscopy (SEM). Therefore, larvae parasitized be fixed in buffered formalin (10%) for histological analysis and buffered glutaraldehyde (2.5%) for scanning electron microscopy. The relationship parasite/host is little studied in fish when compared with parasites of mammals, however it is highly appreciated in international scientific journals, the focus of this project.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALLADAO, G. M. R.; ALVES, L. O.; PILARSKI, F.. Trichodiniasis in Nile tilapia hatcheries: Diagnosis, parasite: host-stage relationship and treatment. Aquaculture, v. 451, p. 444-450, JAN 20 2016. (13/20067-2)