| Grant number: | 13/22962-9 |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| Start date: | February 01, 2014 |
| End date: | January 31, 2015 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal Investigator: | José Maurício Barbanti Duarte |
| Grantee: | Maria Clara Lopes Alvarez |
| Host Institution: | Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil |
Abstract The efficiency of semen cryopreservation is critical for the genomic resource banks construction, which are important tools for maintaining the genetic variability of wild and captive populations, aiming their conservation. One factor that influences the successful of semen cryopreservation is the choice of a cryoprotectant that fits best the characteristics of the ejaculate of a particular species. This study aims to evaluate the efficiency of different penetrating cryoprotectants in the ejaculate of the Mazama americana species. For semen collection, attempts will be made using females in oestrus and the males (n=6) will have the penis deviation for an artificial vagina. After collection, the semen will be divided into three parts, each one will be diluted in a extender containing one of penetrating cryoprotectants to be tested: glycerol (6%), ethylene glycol (3%) and dimethylformamide (5%). To determine which one has the best potential to cryopreserve semen, assessments will be made in pre-freeze and post-thaw samples to determine the vigour, motility, membrane integrity, acrosomal integrity and sperm morphology. | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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