Stem cells expression markers CD44, OCT4 and BMI-1 in oral squamous cell carcinoma

• Grant number: 13/23707-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: January 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Maria Fernanda Setúbal Destro Rodrigues
• Grantee: Camilla Antunes Lopes
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Oral squamous cell carcinoma is the most prevalent neoplasia of oral cavity. The comprehension of this neoplasia is limited regarding its origin and which cells are involved in tumor development and progression, becoming a challenge to the establishment of effective treatment and preventive strategies. In view of this, the identification of neoplastic cells subpopulations on oral squamous cell carcinoma that present the tendency to tumoral expansion, metastasis and alteration in cell death pathways is essential to treatment improvement and survival rates increase of oral squamous cell carcinoma patients. Recently, the discovery that head and neck squamous cell carcinoma comprise a subpopulation of tumoral initiators cells that apparently correspond to malignant stem cells responsible for tumor growth arose the hypothesis of new therapeutic perspectives, as well as the understanding of tumor development mechanisms. Regardless advances on stem cells research, there are few studies focused on the analysis of oral squamous cell carcinoma stem cells. The main purpose of the present study is the isolation and characterization of oral squamous cell carcinoma stem cells from established cell lines using specifics cell molecules. Additionally, it will be analyzed the gene expression profile of genes related to important cell signaling pathways and involved in the regulation of stem cells, and also, their functional hole in controlling essentials cellular processes to malignant transformation contributing to the comprehension of the events involved in the development and evolution of this tumor. Then, diagnostic and therapeutic approaches might become possible after the discovery of potential molecular targets of oral squamous cell carcinoma.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, MARIA FERNANDA SETUBAL DESTRO; XAVIER, FLAVIA CALO AQUINO; ESTEVES, CARINA DUARTE; NASCIMENTO, REBECA BARROS; NOBILE, JULIANA STEPHAN; SEVERINO, PATRICIA; DE CICCO, RAFAEL; TOPORCOV, TATIANA NATASHA; TAJARA, ELOIZA HELENA; NUNES, FABIO DAUMAS. Homeobox gene amplification and methylation in oral squamous cell carcinoma. ARCHIVES OF ORAL BIOLOGY, v. 129, SEP 2021. (13/23707-2)