Serological detection and molecular characterization of agents transmitted by arthropod vectors in wild and domestic animals in the region of the southern Mato Grosso Pantanal

• Grant number: 13/13186-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Rogério André
• Grantee: Keyla Carstens Marques de Sousa
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The diseases transmitted by arthropods have been recently studied in Brazilian wildlife, which can act as hosts for both as vectors for pathogens, many of which have zoonotic potential. The presence of domestic animals in the wild and, vice versa, results in greater human exposure to agents transmitted by arthropods circulating in wild animals. The present study aims to conduct research through serological and molecular agents of the order Rickettsiales (Ehrlichia, Anaplasma and Rickettsia), mycoplasmas hemotróficos, Bartonella spp., Cytauxzoon spp., Hepatozoon and Babesia sp sp. in blood samples from wild and domestic animals (and their ectoparasites) cohabiting the same area of southern Mato Grosso Pantanal. Positive samples in PCR are molecularly characterized by cloning and sequencing to validate the same, compared to sequences already deposited in GenBank and construction of phylogenetic trees. It will also be performed to detect antibodies anti-E. canis, E. chaffeensis, A. phagocytophilum and Rickettsia spp. by Immunofluorescence Assay (IFA) and antibodies anti-B. vogeli by Enzyme-linked immuno sorbent assay (ELISA-test). Additionally will be performed the direct detection of agents through stained blood smears from peripheral capillary puncture tip ear. Thus, the results will contribute to the diagnosis and molecular characterization of the agents which are transmitted by arthropods in southern Mato Grosso Pantanal. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARQUES DE SOUSA, KEYLA CARSTENS; FERNANDES, MARINA PUGNAGHI; HERRERA, HEITOR MIRAGLIA; BENEVENUTE, JYAN LUCAS; SANTOS, FILIPE MARTINS; ROCHA, FABIANA LOPES; GOMES BARRETO, WANESSA TEIXEIRA; MACEDO, GABRIEL CARVALHO; CAMPOS, JOAO BOSCO; MARTINS, THIAGO FERNANDES; et al. Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route. Veterinary Parasitology, v. 237, p. 37-46, APR 15 2017. (15/14896-1, 13/13186-5, 16/10676-0)

DE SOUSA, K. C. M.; CALCHI, A. C.; HERRERA, H. M.; DUMLER, J. S.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; ANDRE, M. R.. Anaplasmataceae agents among wild mammals and ectoparasites in Brazil. EPIDEMIOLOGY AND INFECTION, v. 145, n. 16, p. 3424-3437, DEC 2017. (15/14896-1, 13/13186-5, 15/22387-0)

MARQUES DE SOUSA, KEYLA CARSTENS; FERNANDES, MARINA PUGNAGHI; HERRERA, HEITOR MIRAGLIA; FRESCHI, CARLA ROBERTA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil. TICKS AND TICK-BORNE DISEASES, v. 9, n. 2, p. 245-253, FEB 2018. (15/14896-1, 13/13186-5, 16/10676-0)

MARQUES DE SOUSA, KEYLA CARSTENS; HERRERA, HEITOR MIRAGLIA; SECATO, CAROLINE TOSTES; OLIVEIRA, ANDRE DO VALE; SANTOS, FILIPE MARTINS; ROCHA, FABIANA LOPES; GOMES BARRETO, WANESSA TEIXEIRA; MACEDO, GABRIEL CARVALHO; ESTRELA DE ANDRADE PINTO, PEDRO CORDEIRO; MACHADO, ROSANGELA ZACARIAS; et al. Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland. Acta Tropica, v. 171, p. 172-181, JUL 2017. (16/01216-5, 15/14896-1, 13/13186-5)

MARQUES DE SOUSA, KEYLA CARSTENS; HERRERA, HEITOR MIRAGLIA; ROCHA, FABIANA LOPES; COSTA, FRANCISCO BORGES; MARTINS, THIAGO FERNANDES; LABRUNA, MARCELO BAHIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Rickettsia spp. among wild mammals and their respective ectoparasites in Pantanal wetland, Brazil. TICKS AND TICK-BORNE DISEASES, v. 9, n. 1, p. 10-17, JAN 2018. (15/14896-1, 13/13186-5)

MARQUES DE SOUSA, KEYLA CARSTENS; DO AMARAL, RENAN BRESSIANINI; HERRERA, HEITOR MIRAGLIA; SANTOS, FILIPE MARTINS; MACEDO, GABRIEL CARVALHO; ESTRELA DE ANDRADE PINTO, PEDRO CORDEIRO; BARROS-BATTESTI, DARCI MORAES; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Genetic Diversity of Bartonella spp. in Wild Mammals and Ectoparasites in Brazilian Pantanal. MICROBIAL ECOLOGY, v. 76, n. 2, p. 544-554, AUG 2018. (15/03262-1, 15/14896-1, 13/13186-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUSA, Keyla Carstens Marques de. Serological detection and molecular characterization of agents transmitted by arthropod vectors in wild and domestic animals in the region of the southern mato grosso Pantanal. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.