Identification of sugarcane gene networks and active genome regions associated to drought

• Grant number: 13/13659-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Glaucia Mendes Souza
• Grantee: Danielle Izilda Rodrigues da Silva
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 08/52146-0 - Sugarcane signaling and regulatory networks, AP.BIOEN.TEM
• Associated scholarship(s): 15/01764-0 - Comprehensive analysis of global sugarcane transcriptome changes in response to drought, BE.EP.DR

Abstract

There is a growing environmental and economic importance of sugarcane. Brazil is nowadays the biggest sugarcane producer and also the main producer of sugar and ethanol. In order to maintain a growing sugar and alcohol market, it is expected to expand the sugarcane tillage area to regions with less favorable conditions, such as Brazil Midwest and Northeast regions. Thus, it is important to develop plants with tolerance to stresses, especially drought, as well as to associate this characteristic to high efficiency in ethanol production. Our laboratory is conducting the sugarcane genome sequencing, however, for breeding programs it is also necessary the functional characterization of the identified genes, the understanding of gene regulation and of the functions related to characteristics of agronomical interest. In this context, unveiling the complex sugarcane regulatory networks is a way to obtain information about the regulatory pathways and the possible consequences of its alteration. ChIP-Seq technology has been used in the study of DNA-proteins interactions and has great applicability in the study of transcriptional network regulation. This project aims to obtain a set of enriched genomic sequences in a precipitate of DNA associated to RNA polymerase II in parallel to the definition of a group of genes regulated by water deficit stress. In a long term, the goal of the project is to generate tools that will allow the scientific community to associate regulatory motifs with traits of agronomical interest.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DINIZ, AUGUSTO LIMA; RODRIGUES DA SILVA, DANIELLE IZILDA; LEMBKE, CAROLINA GIMILIANI; LAMAS COSTA, MAXIMILLER DAL-BIANCO; TEN-CATEN, FELIPE; LI, FORREST; VILELA, ROMEL DUARTE; MENOSSI, MARCELO; WARE, DOREEN; ENDRES, LAURICIO; et al. Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 23, DEC 2020. (13/13659-0, 15/01764-0, 17/02270-6, 14/50921-8, 19/15852-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Danielle Izilda Rodrigues da. Comprehensive analysis of sugarcane (Saccharum spp) gene expression changes in response to drought and re-watering conditions. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.