The role of selective and non-selective inhibitors enzyme cyclooxygenase-2 and 5-lipoxygenase enzyme in osteoclastogenesis after the development of experimental periapical

• Grant number: 13/09595-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2014
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Francisco Wanderley Garcia de Paula e Silva
• Grantee: Igor Bassi Ferreira Petean
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis, AP.JP

Abstract

During the inflammatory response, biochemical mediators are released locally in order to stimulate the humoral and cellular immune response. Through the action of the enzyme cyclooxygenase (COX) and lipoxygenase (LO), structural changes occur in the chain of arachidonic acid, leading to the synthesis of these mediators. The objective of this study is to investigate the role of inhibitors of the enzyme 5-lipoxygenase in the treatment of experimental periapical lesions in C57BL / 6. 54 will be used C57BL / 6 mice, six weeks old, weighing from 20 to 22 grams are inoculated in which the root canals of the first molars a solution containing bacterial lipopolysaccharide from E. coli (1.0 mg/ml). After 30 days of induction and as well as the corresponding experimental periods of 7, 14, 21, and 28 days, the animals will be euthanized and the blocks containing the teeth and bone are removed for extraction of total RNA. Then there will be the evaluation of gene expression by a reverse transcription-polymerase chain reaction in real-time (qRT-PCR). The values of the relative expression of each mRNA for each group are compared by analysis of variance (ANOVA) followed by two-way Bonferroni post-test or one-way ANOVA followed by Dunnett's post-test (= ± 0.05). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PETEAN, I. B. F.; ALMEIDA-JUNIOR, L. A.; ARNEZ, M. F. M.; QUEIROZ, A. M.; SILVA, R. A. B.; SILVA, L. A. B.; FACCIOLI, L. H.; PAULA-SILVA, F. W. G.. Celecoxib treatment dampens LPS-induced periapical bone resorption in a mouse model. International Endodontic Journal, v. 54, n. 8, p. 1289-1299, AUG 2021. (10/17611-4, 13/09595-7)

RIBEIRO-SANTOS, FERNANDA REGINA; DA SILVA, GEYSON GALO; FERREIRA PETEAN, IGOR BASSI; MANFRIN ARNEZ, MAYA FERNANDA; BEZERRA DA SILVA, LEA ASSED; FACCIOLI, LUCIA HELENA; GARCIA PAULA-SILVA, FRANCISCO WANDERLEY. Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage. Journal of Applied Oral Science, v. 27, 2019. (13/09595-7, 10/17611-4)

IGOR BASSI FERREIRA PETEAN; ALICE CORRÊA SILVA-SOUSA; TAMARA JUSTINIANO CRONENBOLD; JARDEL FRANCISCO MAZZI-CHAVES; LEA ASSED BEZERRA DA SILVA; RAQUEL ASSED BEZERRA SEGATO; GUILHERME ASSED PIEDADE DE CASTRO; ERIKA CALVANO KUCHLER; FRANCISCO WANDERLEY GARCIA PAULA-SILVA; MANOEL DAMIÃO SOUSA-NETO. Genetic, Cellular and Molecular Aspects involved in Apical Periodontitis. Brazilian Dental Journal, v. 33, n. 4, p. 1-11, 2022-08-26. (13/09595-7, 15/06866-5, 10/17611-4)

PETEAN, IGOR BASSI FERREIRA; SILVA-SOUSA, ALICE CORREA; DA SILVA, RAQUEL ASSED BEZERRA; LUCISANO, MARILIA PACIFICO; DA SILVA, LEA ASSED BEZERRA; DE CASTRO, GUILHERME PIEDADE ASSED; SOUSA-NETO, MANOEL DAMIAO; FACCIOLI, LUCIA HELENA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model. BMC ORAL HEALTH, v. 23, n. 1, p. 9-pg., 2023-01-10. (14/07125-6, 10/17611-4, 13/09595-7)