Proteomics of the Effect of Static and Compensated Magnetic Fields in Cell Proliferation in vitro

• Grant number: 13/24038-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: April 30, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: David Lucas Desidério
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Inserted on the Transdisciplinarity paradigm, by involving interactively diverse areas of knowledge and uniting them for the comprehension of a phenomenon, this research project aims to verify how static and compensated magnetic fields influence the proliferation of undifferentiated mesenchymal stem cells of mice from A/J lineage.This verification will be done with a device constructed to produce a common Static Magnetic Field, with two magnets with its different pole names (North-South) set up to the center. On the contrary, the compensation of magnetic fields will be obtained with six permanent magnets, with equal pole names (North-North), set up to the center in the three orthogonal Cartesian axes. Hypothetically, one hopes that 1- the Static Magnetic Field (SMF: N-S) inhibits the cell proliferation as has been described on the literature. But, with the Compensated Magnetic Field (CMF: N-N) wouldn´t be possible to predict what would happen, since there is no available literature with this experimental design. 2- Primarily one can think of four possibilities in this case: A) No alteration in cell proliferation; B) inhibition of cell proliferation; C) stimulus to cell proliferation; D) Any other observable alterations at protein level. Both cases (SMF and CMF) will be compared to control groups. After, the proteomic analysis will be done to comprehend the intracelular mechanisms involved in the modulation of the proliferation induced by diferente fields, by Ultra Performance Liquid Chromatography and the sequencing of the proteins by Mass Spectrometry.The importance and relevance, as well as justification of this research reside on its future perspectives, contributing with investigations related to tissue engineering and repair, because of the high potencial of tissue differentiation of stem cells. It also can contribute with researches about the influence of magnetic fields on the induction or reduction of tumors by magnetism exposure. Furthermore, the effects of the exposure to these kinds of magnetic fields related to the cellular metabolic pathways can be accessed, contributing to establish safe levels of exposure of living beings to magnetic fields. Nowadays there are many researches about the effect of electromagnetic fields on living beings, but a few that besides this, show the proteomic expression for the understanding of the mechanisms of action of these influences, and less that evaluate comparatively static and/or compensated magnetic fields, thus, justifying this research.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DESIDÉRIO, David Lucas. Proteomics of the effect of compensated and static magnetic fields on cell proliferation in vitro. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.