Analysis and development of a dentin/pulpar biostimulating bandage (BBio)

• Grant number: 13/07836-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2014
• End date: June 30, 2017
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Aparecida de Andrade Moreira Machado
• Grantee: Natalino Lourenço Neto
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of this project is to develop new dentin/pulp capping biostimulator material that may be the basis for the development and recoating of "scaffolds" to repair different dental structures. This study will be composed of two stages: Stage 1 - Development and preparation of a biostimulating bandage (BBio) of dentin and pulpal tissues of deciduous and/or permanent teeth. In this stage, physical and chemical structure tests of the bandage will be carried out. Stage 2 - After defining the physical and chemical properties of BBio, cell culture will be performed in order to study the viability and growth cells of the pulp of human deciduous teeth, and mRNA expression of DMP-1, FGF-2 and Nestin and expression of the DMP-1, FGF-2 and Nestin protein.The techniques used for the analysis will be MTT, RT-PCR and Elisa. The data will be described by means of graphs and tables, by using ANOVA test followed by Tukey test. If necessary, other tests may be applied. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NETO, NATALINO LOURENCO; VITOR, LUCIANA LOURENCO RIBEIRO; DA COSTA, SILGIA APARECIDA; DA COSTA, SIRLENE MARIA; CRUVINEL, THIAGO; OLIVEIRA, THAIS MARCHINI; OLIVEIRA, RODRIGO CARDOSO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA. Physicochemical and biological properties of a biostimulating membrane (BBio) for pulp capping. Materials Letters, v. 308, n. B, FEB 1 2022. (13/07836-7)

ANNELYZE PODOLAN KLOSTER; NATALINO LOURENÇO NETO; SILGIA APARECIDA DA COSTA; THAIS MARCHINI OLIVEIRA; RODRIGO CARDOSO DE OLIVEIRA; MARIA APARECIDA ANDRADE MOREIRA MACHADO. In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel. Brazilian Dental Journal, v. 29, n. 4, p. 354-358, 2018-08-00. (13/07836-7)