Inflammatory citokines involved in oral mucositis and low level lasertherapy on proliferation and migration of gingival fibroblasts

• Grant number: 13/25237-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2014
• End date: August 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Fernanda Gonçalves Basso
• Grantee: Laís Medeiros Cardoso
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Previous studies demonstrated that increased expression of inflammatory cytokines (TNF-±, IL-1² IL-6 e IL-8) is related to mucositis development. The over-expression of these molecules can also delay the tissue healing process. Low-level laser therapy (LLLT) has shown positive clinical effects on oral mucositis lesions and in vitro studies have demonstrated that LLLT can enhance cell metabolism and proliferation. However, there are no data regarding the effects of LLLT on cytokines involved in the ethiopathogenesis of mucositis. Therefore, the aims of this project are to evaluate the effects of: 1) cytokines and LLLT on the wound healing capacity of cultured fibroblasts. Gingival fibroblasts will be cultured and maintained for 48 hours. After that period, a fresh DMEM FBS-free containing the selected inflammatory citokines will be applied on cells, followed by LLLT using a specific prototype - LaserTABLE (InGaAsP - 780±3 nm of wavelength; 25 mW). After the specified treatments, cell proliferation and migration (wound healing assay and transwell assay will be assessed. Data will be evaluated for normality and homocedasticity; after selection of the appropriate statistical tests, a 5% significance level will be considered.