Influence of Hibiscus sabdariffa on the kidney transcriptome analysis - Experimental model of obesity in rats

• Grant number: 14/03045-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2014
• End date: July 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Alessandra Melchert
• Grantee: Andressa Canuto Rosa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This study aims to evaluate the hibiscus sabdariffa supplementation effects on molecular kidney changes in Wistar rats with palatable hyperlipidic diet (PHD) obesity induction. 48 male Wistar rats will be allocated into four groups (n=12): control group (CG)-animals fed with commercial diet for rats and ad libitum water intake; Hibiscus control group (HCG)- animals fed with commercial diet for rats and ad libitum intake of hibiscus in aqueous solution; obese Group (OG)- animals fed with PHD and ad libitum water intake; Hibiscus obese Group (HOG)- animals fed with DPH and ad libitum intake of hibiscus in aqueous solution. Will be used dry extract of hibiscus, diluted in water in a concentration to provide anthocyanins intake of 100-200 mg/kg/day. The animals will receive the diets and hibiscus for 16 weeks. The rats will be weekly weighed and monthly evaluated for food and water intake. A week before the end of the study will be tested for glucose tolerance. At the end of 16 weeks, the animals will be euthanized. Immediately after the death, kidneys will be collect and weigh, then performing right kidney freezing for molecular analysis, to evaluate the kidney transcriptome expression by the transcriptoma shotgun sequencing or RNA-seq technique.