Implementation of molecular techniques for optical and electronic transmission microscopy for the detection of Mycoplasma hyopneumoniae in swine

• Grant number: 14/06459-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2014
• End date: April 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Marcio Hipolito
• Grantee: Luara Lucena Cassiano
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Brazil is in fourth place in the ranking of the world's export of pork, representing 10% of the whole volume exported, reaching a profit of about 1 billion US$ per year. The Swine Enzootic Pneumonia (PES) or Mycoplasmal Swine Pneumonia, caused by the fastidious bacterium Mycoplasma hyopneumoniae, is one of the main respiratory diseases of swine. M. hyopneumoniae destroys the main nonspecific defense mechanism of the respiratory tract, the mucus ciliary elevator, predisposing pigs to secondary pathogens. The aim of this project is to develop techniques of Immunohistochemistry, in situ hybridization and negative staining, immuno-adsorption (ISEM) and immunolabeling with colloidal gold (ME) to detect the presence of M. hyopneumoniae in tissue samples, contributing to further routine tests of pathogenicity in swine and, also, to know the extent of its presence and distribution in the State of São Paulo, and so collaborating with the National Swine Agribusiness.Key-words: Swine enzootic Pneumonia, Mycoplasma, Histopathology, electronic microscopy, in situ hybridization, Immunohistochemistry