Laserphototherapy effects on adhesion and proliferation of human apical papilla stem cells in chitosan sccafold incorporated with blood clot: in vitro study

• Grant number: 14/25281-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2015
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcia Martins Marques
• Grantee: Gabriela Laranjeira Abe
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Young teeth, which have incomplete root formation, when presenting irreversible damage to the pulp tissue have been treated by revascularization. Clinically, the results are encouraging as they show the continuance of root formation and maintenance of tooth vitality. However, histologically there are differences between the newly formed tissue and the original pulp tissue. The cell types that are found and the characteristics of this tissue are not able to perform all the abilities of sound dental pulp. In an attempt to work around this problem, the stem cell homing and the use of biomaterials that support cell adhesion and proliferation, such as scaffolds, facilitate clinical applicability as it eliminates laboratory steps such as in vitro cell culture. The SCAPs are able to differentiate into specific cell types of dental pulp and are recruited into the root canal when the bleeding of periradicular tissues is caused. Chitosan scaffold will be incorporated to stabilize the clot formed, placing it in contact with the dentin internal walls of the root canal that are rich in growth factors which direct cell differentiation. Additionally, to promote the adhesion and proliferation of these cells, laserphototherapy will be associated for its stimulatory role, maintaining cell viability. Therefore, the aim of this study is to evaluate the behavior of SCAPs, as to its adhesion and proliferation when grown in pure chitosan scaffold or incorporated with blood clot, the latter will be yet associated with laserphototherapy or not. Numerical data will be compared statistically by a test to be defined by the analysis of the normality of the results, while the morphology data will be presented in a qualitative and/or illustrative manner. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ABE, Gabriela Laranjeira. Effects of photobiomodulation on adhesion and proliferation of stem cells from human apical papilla in chitosan scaffold with blood clot incorporation. In vitro study. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.