Effect of amniotic fluid presence in cultures of adult and fetal human fibroblasts

• Grant number: 14/25721-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2015
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Cesar Isaac
• Grantee: Leticia Hitomi Yagi
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Scars are the physiological response in adult skin lesions, which can impair the function and aesthetics of the injured area. Fetuses in early gestation, respond to the same damage differently, with skin regeneration. A likely explanation for that difference may be related to factors such as increased type III collagen synthesis by fetal fibroblasts; change in the ratio of beta transforming growth factors 1 and 3 (bTGF3 / bTGF1),due to a reduction of type I collagen; increased production of myofibroblasts (according to the pregnancy phase) and higher capacity of fibroblasts proliferation and migration. The fibroblast growth factor beta (bFGF) a glycoprotein present in the amniotic fluid is related to increase of DNA synthesis and cell biological cycle. bFGF can also promote angiogenesis, tissue remodeling and wound healing. The aim of this study is to compare fibroblasts obtained from human amniotic fluid with fibroblasts from normal adult skin in their in vitro proliferation, collagens type I and III synthesis and capacity of three-dimensional collagen matrix model contraction. Fibroblasts will be obtained from intrauterine amniotic fluid paracentesis of 10 healthy women and fibroblasts from 10 adult skin donor. Cells will be cultured under standard conditions and tested of collagen I, III synthesis capacity, bFGF expression by immunocytochemistry; in vitro cell proliferation and contraction of the 3D matrix model in the presence and absence of amniotic fluid as an enriching factor of the cell culture medium.