Immunohistochemical analysis in alveolar bone tissue of the influence of cisplatin on experimental periodontitis in rats

• Grant number: 15/04639-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Juliano Milanezi de Almeida
• Grantee: Giovani Lopes Fernandes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this animal study will be to evaluate by micro-computed tomography (Micro-CT) and analysis of immunohistochemically study, the influence of chemotherapeutic drug Cisplatin (CIS) on metabolism of alveolar bone in the evolution of experimental periodontitis (EP) in rats. Following a table generated by a computer program, 120 male rats will be divided into 4 groups, with 30 animals each. NEP- SS group, also called the sham group. The animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 0.5 ml of saline solution 0.9 % (SS). These animals will not be induced to EP. EP - SS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 0.5 ml of SS. Soon after the first injection, PE will be induced in the lower first right and left molars. NEP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg of cisplatin (CIS) respectively. These animals will not be induced to EP. EP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg CIS respectively. Soon after the first injection, PE will be induced in the lower first right and left molars. EP was induced by the placement of a number 24 cotton thread around the lower first molars. After of 07, 15 and 30 days after the first intraperitoneal injection (SS or chemotherapy) animals will be euthanized using an overdose of thiopental (150mg/kg). The mandibles collected will be divided into two parts and processed according each analyzes. For the photometric analysis will be evaluated bone loss in the first molar region. The contralateral mandibles are used for immunohistochemical analyzes of biomarkers in the furcation area (RANKL, OPG, TRAP). Quantitative data will be analyzed statistically (p < 0.05) in a specialized computer program (Bioestat 5.0).