Evaluation of the effects of genistein on cell proliferation and differentiation in canine osteosarcomas: in vivo studies.

• Grant number: 15/01538-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2015
• End date: September 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Maria Lucia Zaidan Dagli
• Grantee: Daniel Soares Sanches
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Primary bone tumors of the skeleton are common in dogs, being the osteosarcoma (OSA) the most common bone tumor type. In previous studies, Sanchez, 2013 standardized cultivation, in three dimensions, of canine osteosarcoma cells and proceeded with the chemical induction of cell differentiation in vitro method using genistein. The studies showed induction of partial differentiation of neoplastic osteoblasts and cytotoxic effect besides the control of cell proliferation, and a decrease in the in vitro invasion process. The objective of this project is to verify the effects of treatment with genistein in canine osteosarcoma in vivo. To achieve this, osteosarcoma cell lines (MG63, D17 and CL3) will be inoculated into BALB / c nude mice heterotopically or orthotopically. The animals will, then, receive genistein by oral gavage. The in vivo cell differentiation, as well as its correlation with tumor proliferation, invasion and metastasis differentiation will be evaluated.