Comparison of synthesis methods and scar study endosurgery access in sheep

• Grant number: 15/11611-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2015
• End date: October 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Felipe Farias Pereira da Câmara Barros
• Grantee: Izabela Puerchi Ribeiro
• Host Institution: Coordenadoria Adjunta de Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The aim of the study is to establish a better method and material used to make synthesis in laparoscopic access in sheep, the healing point of view and the technical implementation. The experiment will be conducted with 10 healthy adult sheep underwent laparoscopy. Three 5 mm skin incisions will be made for the entry of laparoscopic portals, respecting the triangulation and pneumoperitoneum, according to the standard technique of laparoscopy in sheep. After exploratory laparoscopy, the synthesis of access shall be in conformity with the established treatment: cutaneous synthesis with Wolff-type points, using surgical nonabsorbable surgical polyamide 2-0 (SN); cutaneous synthesis with intradermal points using absorbable monofilament suture (Polyglecaprona 25) (SA); cutaneous synthesis glue the base of 2-ethyl-cyanoacrylate (SC). This step is timed to reversal of each access, checking the synthesis time for each treatment. The animals should be observed and photographed every day to macroscopic conclusion of which method and material has the best result. 5 days after the procedure half the animals will undergo biopsy of superficial skin and muscle layer in place of the laparoscopic access through 5 mm skin punch for histopathological evaluation. After 10 days after the procedure is done a new biopsy of the other half of the patients. The samples will be processed and stained with hematoxylin-eosin (HE) and trichrome Masson (TM), analyzing vascular proliferation, polymorphonuclear cells, mononuclear cells, fibroblast proliferation and collagen fibers repitelização, assigning the following graduations for this items: absent, mild , moderate and severe.