Effect of the renin-angiotensin system and cyclooxygenase inhibitors on the vascular reactivity in renal hypertensive rat aorta

• Grant number: 15/14113-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2015
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Cardiorenal Pharmacology
• Principal Investigator: Lusiane Maria Bendhack
• Grantee: Luana Rossi Santos
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Renovascular hypertension (2K-1C) is related to the activation of renin-angiotensinall dosteronsystem, production of oxygen reactive species (ROS) induced by angiotensin II and endotelial dysfunction. This project aims to study the vascular relaxation induced by acetylcholine and the contractile response induced by the activation of thromboxane A2receptors (TP) induced by the agonist U46619. We will evaluate the effects of the angiotensin II (AT1) receptor antagonist (Losartan) on the relaxation and contraction responses. We will also evaluate the effects of the inhibitors of COX (ibuprofen) and angiotensin converting enzyme (captopril) in intact endothelium and denuded rat aortas. Our study will be performed in normotensive (2K) and 2K-1C hypertensive rat aorta. The hypothesis of this study is that in renal hypertensive rat aorta, the relaxation induced by acetylcholine and contraction induced by U46619 are modulated by AT1 activation by angiotensin II, which is produced by tissue angiotensin converting enzyme.