Effects of vitamin C supplementation on productive performance and tissue regeneration of the zebrafish (Danio rerio)

• Grant number: 15/13798-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2015
• End date: November 30, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Margarida Maria Barros
• Grantee: Pedro Henrique Ventura de Almeida
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This study aims to evaluate the growth performance and fin regeneration rates of juvenile zebrafish fed diets containing increasing levels of vitamin C. A basal semi-purified diet will be formulated to test six levels of ascorbic acid supplementation (0, 75, 150, 300, 600 and 1200 mg kg-1). A total of 900 juvenile zebrafish (28 days post hatch) will be randomly distributed in 30 15 L aquariums, constituting an experimental design with six treatments and five replications. After a 60-day feeding trial, the growth performance will be evaluated. The fish will be captured and anesthetized for assessing weight gain, medium length, feed intake, feed conversion, specific growth rate and survival rate. To analyze the regeneration rates of the caudal fin 12 animals of each treatment will be anesthetized and with the aid of a scalpel will have part of their fins amputated. To determine the regenerated portion on days 7, 14 and 21 after amputation, four animals of each treatment will be sacrificed and then subjected to image analysis system Leica Qwin v.3.0. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PUCCI FIGUEIREDO CARVALHO, PEDRO LUIZ; VENTURA ALMEIDA, PEDRO HENRIQUE; XAVIER, WILLIAM DOS SANTOS; TIAGUA VICENTE, IGOR SIMOES; GUIMARAES, MATHEUS GARDIM; DAMASCENO RODRIGUES, EDGAR JUNIO; ITO, PAULO INCANE; PEZZATO, LUIZ EDIVALDO; BARROS, MARGARIDA MARIA. Vitamin C supplementation improves growth performance and caudal fin regeneration in zebrafish (Danio rerio). AQUACULTURE NUTRITION, v. 27, n. 1, NOV 2021. (15/13798-6)