Influence of chemotherapy on expression of pro inflammatory cytokines tissue in experimental periodontitis

• Grant number: 15/21332-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2015
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Juliano Milanezi de Almeida
• Grantee: Clara Possarle Cunha
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study in animals is to evaluate by comparative analysis, by the biochemical and immunohistochemical methods, the influence of Cisplatin on the evolution of experimental periodontitis (EP) in rats. 120 male rats will be set into 4 groups with 30 animals each. NEP-SSgroup also called sham group. The animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 0.5 ml of saline solution 0.9 % (SS). These animals will not be induced to EP. EP- SSgroup: the animals in this group will receive two intraperitoneal injections with 48-hourinterval between them of 0.5 ml of SS. Soon after the first injection EP will be induced in the lower first right and left molars. NEP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg of cisplatin (CIS) respectively. These animals will not be induced to EP. EP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg CIS respectively. Soon after the first injection EP will be induced in the lower first right and left molars. EP will be induced by the placement of a number 24 cotton thread around the lower first molars. After 07, 15 and 30 days from the first intraperitoneal injection (SS or CIS) animals will be euthanized using an overdose of thiopental (150mg/kg) associated to Xilasin. Blood will be collected via cardiac puncture for biochemical analysis at the following times: prior the application of drug or SS and at the euthanasia periods (7, 15 and 30 days). Blood samples will be used for biochemical analysis of creatinine and urea. The mandibles will used for immunohistochemically analysis of biomarkers in the furcation area of the lower first molars (TNF± and IL1²). Quantitative data will be analyzed statistically (p < 0.05).