Skin tumors with sebaceous differentiation and its mimickers: immunohistochemical and histochemical evaluation of selected cases

• Grant number: 15/19494-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2016
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Maria Letícia Cintra
• Grantee: Giovanna Guzzo
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Tumors with sebaceous differentiation comprise a heterogeneous and unusual group of skin adnexal lesions. The classification of these tumors includes hyperplastic, hamartomatous and benign or malignant lesions. Sebaceous tumors, similarly to sebaceous glands, often display sebocytes with multivacuolated clear cytoplasm, a characteristic finding of sebaceous differentiation, However, non-sebaceous tumors can present cells with a very similar morphology, as squamous and basal cell carcinoma, xanthelasma, trichilemmoma, hidradenoma and melanocytic lesions, among others. Distinguishing and classifying these tumors is crucial since prognosis and treatment are different. Histochemical and immunohistochemical staining could assist in this task, but there are relatively few immunomarkers described as useful in their histological evaluation and, for some of these markers, only reports evaluating small number of cases. We intend to analyze cases of sebaceous tumors and clear cell tumors mimicking sebaceous differentiation, with the aim of adding information for improving differential diagnosis of these tumors.. The material was already selected from the Pathology files of Unicamp Clinics Hospital. PAS stain and a broad panel of immunohistochemical antibodies will be used, including those commonly used to set sebaceous differentiation and others not yet described in this group of tumors (EMA, adipophilin, perforin, androgen receptor, CK7, CK8 / 18, CK15, CK19, Ki-67, MCM3, p21, p53, bcl-2, CD117, MSH2, MSH6, MLH1, PMS2).