Analysis of inflammatory cells in the bronchoalveolar lavage after lung transplantation in rats with creatine supplementation

• Grant number: 15/19885-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2015
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Rogerio Pazetti
• Grantee: João Vitor Moraes Pithon Napoli
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The ischemia and reperfusion (IR) is a complication that can raise the risk of death after lung transplantation, by activating the innate immune system to induce inflammation. This occurs when the supplemental oxygen in the lungs is interrupted (ischemia) followed by reperfusion period. During ischemia, oxygen supply is beneath metabolic needs, resulting in depletion of cellular ATP reserves and increasing production of reactive oxygen (ROS) and nitrogen (RNS) species. The ROS and RNS are able to react with and modify the molecular structures of lipids, carbohydrates, proteins, deoxyribonucleic acid and leading to structural and functional damage to cells. In addition, the IR also triggers an intense inflammatory process, mainly characterized by the presence of neutrophils and activated macrophages, which release several inflammatory mediators, perpetuating inflammation. The lock of each of these steps is an attempt to alleviate the injury, preventing the dysfunction of organs involved in the IR, particularly the lungs. This knowledge has evolved along with the study of antioxidants capable of neutralizing the effects of ROS and RNS.