Pneumoperitonium effects: prospective, double-blind and randomized experimental study with comparative lung morphologic variations

• Grant number: 15/07221-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2016
• End date: August 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Itamar Souza de Oliveira-Júnior
• Grantee: Carla Andréia Dato
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Minimally invasive surgery, such as laparoscopy, causing minor trauma and attenuate the stress response. Moreover, the production and degradation of oxidative stress and inflammatory mediators are nonetheless an integral part of the responses to surgery. However pneumoperitoneum or pneumoretroperitoneum remains involved in the imbalances of stress response, by augment of inflammatory cels, production of free radicals and cytokines. Purpose: Our study aims to assess the effects of pneumoperitoneum in the lungs of animals by insufflation of carbon dioxide (simulating a laparoscopy). Methods: use of male animals divided in five groups (n = 6 per group), following: Sham: without intra-abdominal insufflations; IAP5: using intra-abdominal pressure of 5 mmHg carbon dioxide; IAP8: using intra-abdominal pressure of 8 mmHg carbon dioxide; IAP10: using intra-abdominal pressure of 10 mmHg carbon dioxide; IAP12: using intra-abdominal pressure of 12 mmHg carbon dioxide; and IAP14: using intra-abdominal pressure of 14 mmHg carbon dioxide. At the end of the experiment will perform the euthanasia of animals and we will remove the lungs for histological analysis. Statistical analysis will be made using the descriptive analysis provided by a pathologist (blind analysis).