Papain permeability evaluation in oral pharmaceuticals formulations for treatment of intestinal diseases triple co-culture of Caco-2, HT29-MTX and Raji cells as in vitro model

• Grant number: 15/19212-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Patricia Santos Lopes
• Grantee: Fúlvio Gabriel Corazza
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Currently the production of medicines containing enzymes have been promising due to the magnitude of their therapeutic potential. Papain is a proteolytic enzyme isolated from the latex of leaves and green fruit of papaya adult (Carica papaya Linné) widely used as a debridement agent in the processes of healing and treatment of wounds mainly due to its ability to promote tissue growth in addition to being employed as a promoter of oral and dermal absorption. Although widely used in topical formulations, papain is underexplored in oral use formulations for the treatment of intestinal diseases. The development of new medicinal products for oral use, the tests that evaluate the permeability and the toxicity of drugs throughout the gastrointestinal tract are very important, however, for bioethical issues, it is necessary to reduce the use of animals in the framework of scientific research for this purpose. In this project, will be evaluated the permeability of oral use formulations containing papain to intestinal pathologies treatment using in vitro models biomimetics of co-cultures of Caco-2, HT29-MTX and Raji cells. The results will be disseminated and should contribute in an innovative way with scientific research and development.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORAZZA, FULVIO G.; ERNESTO, JULIA V.; NAMBU, FELIPE A. N.; CALIXTO, LEANDRO A.; VARCA, GUSTAVO H. C.; VIEIRA, DANIEL P.; LEITE-SILVA, VANIA R.; ANDREO-FILHO, NEWTON; LOPES, PATRICIA S.. Enhancing the Furosemide Permeability by Papain Minitablets Through a Triple Co-culture In Vitro Intestinal Cell Model. AAPS PHARMSCITECH, v. 21, n. 7, p. 10-pg., 2020-09-04. (10/10935-9, 16/22916-5, 15/19213-0, 15/19212-3)

CORAZZA, FULVIO G.; ERNESTO, V, JULIA; NAMBU, FELIPE A. N.; DE CARVALHO, LUMA R.; LEITE-SILVA, VANIA R.; VARCA, GUSTAVO H. C.; CALIXTO, LEANDRO A.; VIEIRA, DANIEL P.; ANDREO-FILHO, NEWTON; LOPES, PATRICIA S.. Papain-cyclodextrin complexes as an intestinal permeation enhancer: Permeability and in vitro safety evaluation. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 55, FEB 2020. (15/19212-3, 10/10935-9, 16/22916-5, 15/19213-0)