Unveiling the role of HSPB1 in PRL-induced autophagy inhibition in beta-cells

• Grant number: 16/07470-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2016
• End date: April 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Leticia Labriola
• Grantee: Fábio Fernando Alves da Silva
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Autophagy is a mechanism of recycling cytoplasmic components and is essential for cellular homeostasis. Under stress conditions, this mechanism is activated above basal levels, promoting the degradation of protein aggregates and defective organelles, thus avoiding cell damage that could compromise cell viability. Studies carried out by our group have shown not only that PRL promotes cytoprotection in beta-cells, reducing pro-inflammatory cytokines-induced apoptosis, but also that HSPB1 plays an essential role in this inhibition of apoptosis mediated by PRL after treatment with cytokines. Moreover, recent results from our laboratory showed an increase in autophagy levels in beta-cells after exposure to cytokines, as well as a restauration to normal levels in the presence of PRL. In order to better understand the role of PRL in the inhibition of autophagy in these cells, the aim of this project is to study whether HSPB1 is also essential in the mechanism of autophagy regulation induced by PRL. The results generated in this study may help to increase the knowledge of the molecular events induced by PRL in beta-cells, and may allow to infer new approaches to improve cytoprotection, culture and transplantation of these cells into type 1 diabetic patients. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Fábio Fernando Alves da. Unveiling the role of HSPB1 in PRL-induced autophagy modulation in beta-cells. 2018. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.