Association of a nanofiber scaffold modified by cold atmospheric plasma with DPSC in regenerative endodontics

• Grant number: 16/06188-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2016
• End date: December 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Carlos Henrique Ribeiro Camargo
• Grantee: Felipe de Toledo Amici
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Tissue engineering has shown some promising methods to restore vitality of thedental pulp, beeing and it's essencial to its progress the development of scaffoldsand the incorporation of new technologies. Thus, nanofibers scaffolds composedof polymeric substances generated by eletrospinning have optimistic results inregenerative endodontics. However, the lack of cell recognition and low surfacefree energy of these scaffolds are factors that prejudice the adhesion,proliferation and differentiation. In this project, cold plasma at atmosphericpressure will be used to change and increase the reactivity of a polidioxanone /hexaisofluoroisopropanol scaffold with LL-37, evaluating whether this treatmentwill promote an increase in the surface free energy by measuring the contactangle with a goniometer and analyzing its results in vitro quantitatively andqualitatively in dental pulp stem cells (DPSC) proliferation by scanning electronmicroscope. The results will be analyzed by statistical tests previously chosen, itwill be applied the most convenient test for each analysis using significance levelof 5%.