Proteomic analysis of ejaculated and epididymal sperm plasma membrane in stallions

• Grant number: 16/12375-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Priscilla Nascimento Guasti
• Grantee: Juliana Pedroso Mendes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

During ejaculation, the proteins present in the secretion of the accessory glands interact with the surface of the sperm membrane, changing its structure and even its functionality. This study aims to quantify and identify the proteins of the plasma membrane of sperm recovered from the cauda epididymis and ejaculated of stallions. Eight stallions will be submitted to semen collection and then bilateral orchiectomy for recovery spermatozoa from the cauda epididymis. The samples will be submitted to extraction of the sperm membrane proteins and protein quantification. Subsequently, epididymal and ejaculated sperm samples will be submitted to one-dimensional electrophoresis, differen