Characterizing the relation between the Tnt1 retrotransposon expression and Nicotiana tabacum's cell cycle

• Grant number: 16/12454-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2016
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Marie-Anne Van Sluys
• Grantee: Ana Paula Muche Schiavo
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The transposable element Tnt1 is present in a high number of copies in the Nicotiana tabacum genome. In previous results, this transposable element was indicated to be linked with development of leaf-disc-regenarated tobacco. Also in previous results another transposable element from the same family as Tnt1 appears to have a cyclic pattern of expression throughout the cell cycle. Thus, this project aims to characterize the relation between Tnt1 expression and the cell cycle with silencing studies and expression profiling of Tnt1 from Bright Yellow 2 (BY-2) tobacco cells.