Participation of bone morphogenetic protein 9 (BMP-9) in the interaction between osteoblasts and titanium with nanotopography

• Grant number: 16/14477-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2016
• End date: October 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Márcio Mateus Beloti
• Grantee: Barbara Luana da Silva Bezerra
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Bone morphogenetic proteins (BMPs) are cytokines that belong to the transforming growth factor-² (TGF-²) superfamily and are involved in several biological processes, including osteoblast differentiation and bone formation, both events directly associated with osseointegration of titanium (Ti) implants. Our research group demonstrated that the osseoinductor effect of a Ti with nanotopography, generated by H2SO4/H2O2 treatment, is due to, at least in part, BMP signaling pathway modulation. In this context, the aims of this study are to (1) investigate the participation of exogenous BMP-9 in the interaction between osteoblastic cells and Ti with nanotopography, compared with machined Ti and (2) investigate the effect of Ti with nanotopography on the endogenous BMP-9, compared with machined Ti. The MC3T3-E1 pre-osteoblastic cell line will be cultured in presence of different BMP-9 concentrations (0 nM - vehicle, 0.1 nM, 1 nM and 10 nM) and alkaline phosphatase (ALP) activity and extracellular matrix mineralization will be evaluated in order to determine the concentration with higher osteogenic potential. Then, MC3T3-E1 cells will be cultured on Ti with nanotopography and machined Ti with or without BMP-9 at the previously selected concentration and the same osteoblast parameters will be evaluated. Additionally, MC3T3-E1 cells will be cultured on Ti with nanotopography and machined Ti without BMP-9 and the gene expression of endogenous BMP-9 will be evaluated. The results will show (1) if cells grown on Ti with nanotopography are more responsible to exogenous BMP-9 and (2) if Ti with nanotopography stimulates cells to synthesize higher amount of endogenous BMP-9. The outcomes of this study could contribute to clarify the intracellular mechanisms involved in the interaction between bone tissue and Ti implants and consequently to the development of novel strategies involving BMPs and nanotopography in order to favor the process of osseointegration.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, ALANN T. P.; BEZERRA, BARBARA L. S.; OLIVEIRA, FABIOLA S.; FREITAS, GILEADE P.; BIGHETTI TREVISAN, RAYANA L.; OLIVEIRA, PAULO T.; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Effect of bone morphogenetic protein 9 on osteoblast differentiation of cells grown on titanium with nanotopography. Journal of Cellular Biochemistry, v. 119, n. 10, p. 8441-8449, NOV 2018. (16/14171-0, 16/14477-1)