Directed evolution of P. aeruginosa in continuos culture

• Grant number: 16/19450-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2016
• End date: November 30, 2019
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Beny Spira
• Grantee: Vinicius Sousa Flores
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Phosphorus is an important element in the formation and metabolism of living beings. The genome Bacterial encoding various proteins involved in the uptake and phosphate metabolism(Pi), collectively known as regulon PHO. Pi transport in bacteria is mediated for more than one protein system. Pst is a high affinity Pi transport system. It was first described in E. coli and other bacteria then. It is encoded by an operon of the same name, and its induced expression in low Pi concentrations in the environment. The Pst system has a second function, which is to suppress the PHO regulon in situations abundance of Pi. Pseudomonas aeruginosa is a gamma-proteobacteria present in several environments, including those extremely oligotrophic. This proposal aims to study the evolutionary process of P. aeruginosa under strong phosphate limitation and isolate bacteria capable Pi uptake increased by evolution technique directed in culture continuous chemostat. (AU)