High-risk HPV DNA detection and analysis of the mutational status of KRAS and PIK3CA genes and inflammatory cytokines panel in patients with esophageal cancer and megaesophagus with and without cancer

• Grant number: 15/20077-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2017
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Adhemar Longatto Filho
• Grantee: Fernanda Franco Munari
• Host Institution: Hospital do Câncer de Barretos. Fundação Pio XII (FP). Barretos , SP, Brazil
• Associated scholarship(s): 17/05788-6 - Mutational profile of esophageal cancer associated or not with chagasic megaesophagus in Brazilian patients., BE.EP.MS

Abstract

Esophageal cancer (EC) is the eighth most common cancer and the sixth most common cause of death worldwide, with 80% of cases occurring in developing countries such as Brazil, which are considered high-risk countries for the occurrence of the disease. In Brazil, EC is one of the ten most frequent types of cancers, occupying 6th place in men and 9th for women. Among the risk factors for squamous cell carcinoma of esophagus (ESCC), smoking, alcohol consumption, the chagasic megaesophagus (MEC) stands out and, more recently, the participation of human papillomavirus (HPV) is being questioned. Chagas disease (CD) caused by Trypanosoma cruzi parasite occurs mainly in South and Central America and Mexico and Brazil; The chagasic disease occur as acute or chronic phase, the latter being more often in the heart and digestive tract. The MEC is a common digestive manifestation of CD which consists in the not harmonic opening of the upper esophageal sphincter that causes the increase in diameter of the organ due to ineffective esophageal propulsion and food stasis that favor the development of esophageal cancer. However, there are still no reports that demonstrate the relationship between the presence of HPV, EC and MEC. However, mutations in PIK3CA and KRAS gene are important for the development and tumor maintenance. Moreover, inflammation is a process associated with several changes in the cellular and molecular components in which cytokines play an important role in the development and tumor progression can be considered as a biological marker in EC, therefore, important to identify a panel cytokines in patients with EC as well as in patients with MEC with and without cancer. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNARI, FERNANDA FRANCO; DOS SANTOS, WELLINGTON; EVANGELISTA, ADRIANE FEIJO; CARVALHO, ANA CAROLINA; PASTREZ, PAULA AGUIAR; BUGATTI, DIEGO; WOHNRATH, DURVAL R.; SCAPULATEMPO-NETO, CRISTOVAM; GUIMARAES, DENISE PEIXOTO; LONGATTO-FILHO, ADHEMAR; et al. Profile of esophageal squamous cell carcinoma mutations in Brazilian patients. SCIENTIFIC REPORTS, v. 11, n. 1, OCT 18 2021. (15/20077-3)

MUNARI, FERNANDA FRANCO; SICHERO, LAURA; CARLONI, ADRIANA CRUVINEL; LACERDA, CROIDER FRANCO; NUNES, EMILY MONTOSA; TORRES DE OLIVEIRA, ANTONIO TALVANE; SCAPULATEMPO-NETO, CRISTOVAM; MORINI DA SILVA, SANDRA REGINA; CREMA, EDUARDO; ADAD, SHEILA JORGE; et al. requency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinom. PATHOBIOLOGY, OCT 2021. (15/20077-3)

MUNARI, FERNANDA FRANCO; CARLONI, ADRIANA CRUVINEL; MARIANO, VANIA SAMMARTINO; SYRJANEN, KARI; REIS, RUI MANUEL; LONGATTO-FILHO, ADHEMAR. The relationship between esophageal cancer, chagasic megaesophagus and HPV: myths, tales or reality?. HISTOLOGY AND HISTOPATHOLOGY, v. 33, n. 11, p. 1135-1149, NOV 2018. (15/20077-3)

ROSA, MARCELA NUNES; EVANGELISTA, ADRIANE FEIJO; LEAL, LETICIA FERRO; DE OLIVEIRA, CRISTINA MENDES; OLIVEIRA SILVA, VIVIANE ALINE; MUNARI, CARLA CAROLINA; MUNARI, FERNANDA FRANCO; MATSUSHITA, GRAZIELA DE MACEDO; DOS REIS, RICARDO; ANDRADE, CARLOS EDUARDO; et al. Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line. SCIENTIFIC REPORTS, v. 9, FEB 13 2019. (15/20077-3)

MUNARI, FERNANDA FRANCO; CRUVINEL-CARLONI, ADRIANA; LACERDA, CROIDER FRANCO; TORRES DE OLIVEIRA, ANTONIO TALVANE; SCAPULATEMPO-NETO, CRISTOVAM; MORINI DA SILVA, SANDRA REGINA; CREMA, EDUARDO; ADAD, SHEILA JORGE; MARCHESAN RODRIGUES, MARIA APARECIDA; COELHO ARRUDA HENRY, MARIA APARECIDA; et al. PIK3CA mutations are frequent in esophageal squamous cell carcinoma associated with chagasic megaesophagus and are associated with a worse patient outcome. INFECTIOUS AGENTS AND CANCER, v. 13, DEC 29 2018. (15/20077-3)