Bacterial cellulose production using different substrates for use in sustained release system for antibacterial agents

• Grant number: 16/17278-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2016
• End date: November 30, 2017
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Wilton Rogério Lustri
• Grantee: Caroline Yamada
• Host Institution: Universidade de Araraquara (UNIARA). Associação São Bento de Ensino. Araraquara , SP, Brazil

Abstract

Cellulose is an insoluble biopolymers more abundant water found in nature and, although plants constitute the most important source of this polymer, with an estimated annual production 1010-1011 tons / year can be produced for many types of organisms, including bacteria . The cellulose derived from plant is a pulp which is associated with other types of natural fibers such as lignin and hemicellulose, while the bacterial cellulose (BC) is free of such fibers. Since its discovery, the CB has proved to be a biopolymer of great interest for use in various industrial and medical fields due to their different physical properties of plant cellulose, among which stand out the mechanical strength, hydrophilicity, high porosity and biocompatibility. CB can be produced by various bacterial species, including Sarcina genus, Agrobacterium, Rhizobium, Acetobacter and Gluconacetobacter especially by bacteria of the genus, especially the species G. and G. xylinus hansenii, using a variety of natural and synthetic culture medium with different nutrient sources. However, there is relatively difficult to obtain CB-scale, using the culture media and conventional systems due to its low production yield. Thus, the study of new production methods and culture conditions allowing obtaining, on a large scale and the lowest possible cost becomes extremely important, since the CB is a biopolymer with high potential for use by the pharmaceutical industry and biomedical for use in transdermal compositions and for topical use. This research project aims to use different composition of culture media, using supplementation with additional substrates and varying carbon sources, to optimization of bacterial cellulose production by G. hansenii ATCC 23769 in comparison to other means cultivation in the published literature, besides the characterization physicochemical, structural determination and production yield. Is also objective of the study, analysis of retention capacity and sustained release of antibacterial agents. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LAZARINI, S. C.; YAMADA, C.; BARUD, H. S.; TROVATTI, E.; CORBI, P. P.; LUSTRI, W. R.. Influence of chemical and physical conditions in selection of Gluconacetobacter hansenii ATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports. Journal of Applied Microbiology, v. 125, n. 3, p. 777-791, SEP 2018. (15/09833-0, 15/25114-4, 16/17278-0)