In vivo characterization of the regulatory activity of Bacillus subtilis purine riboswitches

• Grant number: 17/02594-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2017
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Danielle Biscaro Pedrolli
• Grantee: Nathan Vinícius Ribeiro
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 14/17564-7 - Reprogramming the purine metabolism of Bacillus subtilis employing sRNA-technology, AP.JP

Abstract

In the first report related to the JP project, the regulatory activity of the Bacillus subtilis purine riboswitches were characterized in vitro. sRNAs designed to target these riboswitches were also assayed in vitro to access their functionality. Next, sRNAs functionality will be assayed in vivo using B. subtilis. Therefore, it is necessary to characterize the riboswitches in vivo first. (AU)